Intended Use:
A method control is provided to customers when positive materials are unavailable. The method control is made of anti-animal antibodies and does not contain any virus coat protein.
This control is approved for use in the following ELISA test systems: Grapevine fanleaf virus (GFLV), Leek yellow stripe virus (LYSV), Onion yellow dwarf virus (OYDV), Peach rosette mosaic virus (PRMV), and Potato virus T (PVT).
Lyophilized controls should be reconstituted with the sample extract buffer designated in your test user guide.
Includes:
- Vial of lyophilized control material.
- Makes 2 mL final solution.
- User Guide
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希望同路人多多交流啊!
这些染料都非常成熟,光毒和淬灭都很低,当然要考虑到你采集图像时的显微镜参数。
比如calcein,常用的示踪,绿光(虽然这些颜色只是根据光谱加上去的伪彩),但要考虑你的实验过程中结合细胞结构,是否会伴随calcein的泄漏,就是荧光降低。
dri,还可以看膜啊
cfda也不错
bcef虽是ph指示,但你试验时不仅可观察细胞,还可看细胞ph变化,也行。
当然你或许还要结合其他方法,如细胞免疫化学等手段去双染或多染,都需要综合考虑染料之间特性。单独染一个染料,有点浪费,不如多染,数据和图像也好看些。现在流行细胞成像。

