Prolume Purple exhibitsanupto13-foldhigherluminescencethanCTZ400a(“deepblueC”).
Pleaseseeour"Howtouse"forfurtherinformation.
| CompoundID: | ProlumePurple(PP-CTZ) |
| alternativename: | methoxye-Coelenterazine(me-O-eCTZ) |
| MolecularFormula: | C29H25N3O3 |
| MW: | 463.53 |
| Structure: | ![]() |
| Application: | emitsaround405nmwithRLuc,RLucII,RLuc3,RLuc8andGreenRenillaLuc |
| Solvent: | WerecommendNanoFuelSolvent.LowsolubilityinEthanol. |
| comparisontoCTZ400a: | ![]() |
| EmissionspecwithRenillaLuciferases: | ![]() |
| References: | BioluminescenceAssistedSwitchingandFluorescenceImaging(BASFI) ProlumePurple(Cat.#369)wasenablingtheseresearchestofollowproteinmovementwithinasinglecellinreal-timeforupto20minusingasophisticatedBRETsystem: MonitoringGprotein-coupledreceptorandbeta-arrestintraffickinginlivecellsusingenhancedbystanderBRET YoonNamkung,ChristianLeGouill,ViktoriaLukashova,HiroyukiKobayashi,MireilleHougue,EtienneKhoury,MideumSong,MicharlBouvier,StephaneA.Laporte |
HowtouseProlumePurple
CertificateofAnalysis HPLC MSDS NMR
Theluciferinisshippedasfiltered,lyophilized,batchcontrolledsubstrate in250ugand500ugaliquots.
1mg=2x500ug. 10mgsispackedasregularpowderinonetube.
Theadvantagesoflyophilizedluciferinsare:
- pre-aliquotedinsmallamounts,freshsubstrateforeverysetofexperiments
- longshelf-life(packedunderArgon)
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
希望同路人多多交流啊!
这些染料都非常成熟,光毒和淬灭都很低,当然要考虑到你采集图像时的显微镜参数。
比如calcein,常用的示踪,绿光(虽然这些颜色只是根据光谱加上去的伪彩),但要考虑你的实验过程中结合细胞结构,是否会伴随calcein的泄漏,就是荧光降低。
dri,还可以看膜啊
cfda也不错
bcef虽是ph指示,但你试验时不仅可观察细胞,还可看细胞ph变化,也行。
当然你或许还要结合其他方法,如细胞免疫化学等手段去双染或多染,都需要综合考虑染料之间特性。单独染一个染料,有点浪费,不如多染,数据和图像也好看些。现在流行细胞成像。




