AmplideX® PCR/CE HTT Kit
The AmplideX PCR/CE HTT Kit* makes the detection and sizing of CAG trinucleotide repeats in the HTT gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA.
Features & Benefits
The AmplideX PCR/CE HTT Kit includes reagents to perform a two-primer, anchor-primed PCR of the HTT CAG repeat region followed by fragment sizing by capillary electrophoresis. This single-tube PCR reaction provides a streamlined workflow, enabling sample-to-result in approximately 6 hours using readily available laboratory equipment.
Reduced Complexity
- Proprietary PCR solutions for GC-rich amplification and detection
- Eliminates need for multiple PCRs – one result, straightforward analysis
- Resolves zygosity and detects large expansions
Optimized Workflow
- Fully kitted, end-to-end solutions that significantly reduce hands-on-time
- Sample-to-result possible within a single shift
- Identical PCR and CE conditions as the AmplideX PCR/CE DMPK Kit
Quality Results
- Reliable, unambiguous results and a robust stutter peak pattern
- Accurate sizing across the entire CAG repeat range
- Software converts raw base pair data into number of repeats
*For Research Use Only. Not for use in diagnostic procedures.
Analytical Characteristics
- Excellent Concordance with Previously Characterized Samples (Table 1)
- Repeat profile is conserved across known SNPs (Figure 1)
- Clearly resolves zygosity (Figure 2)
Table 1. Comparison of CAG repeat alleles in commercially available samples, as reported by Kalman et al. (2007)
Figure 1. Repeat profile is conserved across known SNPs.
Figure 2. Zygosity is clearly resolved using the AmplideX PCR/CE HTT Kit.
Figure 3. Workflow for the AmplideX PCR/CE HTT Kit.
Ordering Information
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| AmplideX® PCR/CE HTT Kit (RUO) | 32 | 49657 |
T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com
AmplideX® PCR/CE HTT Kit
The AmplideX PCR/CE HTT Kit* makes the detection and sizing of CAG trinucleotide repeats in the HTT gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA.
Features & Benefits
The AmplideX PCR/CE HTT Kit includes reagents to perform a two-primer, anchor-primed PCR of the HTT CAG repeat region followed by fragment sizing by capillary electrophoresis. This single-tube PCR reaction provides a streamlined workflow, enabling sample-to-result in approximately 6 hours using readily available laboratory equipment.
Reduced Complexity
- Proprietary PCR solutions for GC-rich amplification and detection
- Eliminates need for multiple PCRs – one result, straightforward analysis
- Resolves zygosity and detects large expansions
Optimized Workflow
- Fully kitted, end-to-end solutions that significantly reduce hands-on-time
- Sample-to-result possible within a single shift
- Identical PCR and CE conditions as the AmplideX PCR/CE DMPK Kit
Quality Results
- Reliable, unambiguous results and a robust stutter peak pattern
- Accurate sizing across the entire CAG repeat range
- Software converts raw base pair data into number of repeats
*For Research Use Only. Not for use in diagnostic procedures.
Analytical Characteristics
- Excellent Concordance with Previously Characterized Samples (Table 1)
- Repeat profile is conserved across known SNPs (Figure 1)
- Clearly resolves zygosity (Figure 2)
Table 1. Comparison of CAG repeat alleles in commercially available samples, as reported by Kalman et al. (2007)
Figure 1. Repeat profile is conserved across known SNPs.
Figure 2. Zygosity is clearly resolved using the AmplideX PCR/CE HTT Kit.
Figure 3. Workflow for the AmplideX PCR/CE HTT Kit.
Ordering Information
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| AmplideX® PCR/CE HTT Kit (RUO) | 32 | 49657 |
T 1-877-777-1874; 512-681-5200 F 1-512-681-5202 E orders@asuragen.com
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而且样品中的无水硫酸钠未变色,而做标准曲线的五个和空白对照的变为蓝色了,请高手指教,多谢!
还有,是否变蓝对测定结果有影响吗?
谢了哈
欢迎你！请下次规范发贴:)
健那绿——高中唯一一个活体染色剂。染线粒体的，染成蓝绿色
健那绿染液是一种活体染液，实验对象必须是活细胞，健那绿可以使活细胞中的线粒体呈现蓝绿色，而细胞质接近无色。
请各位大侠给予帮助！！
谢谢！！
产品主要应用：点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。
相关产品如下：
中文名英文名产品编号分子结构Cy7.5胺Cy7.5amineAGF1350A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110804_5250.jpg[/img]Cy5胺Cy5amineAGF1332A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110715_7750.jpg[/img]相似系列产品：
抗体、核酸、蛋白质等生物分子标记染料
羰基活性荧光染料
巯基反应性染料
羧酸类染料
1、快讯上是Invotrogen公司的SYRBGOLD，不过500ul/1390元，实在有点贵，为了查用量；
2、结果又查出赛百盛的GoldViewTMDNA染料1ml/100元；
3、还有一种在日光下即可看出DNA条带的上海华舜生物工程有限公司的LightBluedye；
为了一个EB，居然不小心查出三种染料，而对这三种染料的评价也褒贬不一，我个人希望找个毒害小些，而且灵敏度还不能低于EB，价格方面也能让老板接受的来。
而对这三个染料，我有三个问题：
1、SYRBGOLD的用量问题，500ul能用到200次吗（我一般一次用30ml的胶，配在胶中）？
2、GoldViewTMDNA染料的成分是否如有些人所说的就是丫啶橙（能举出确切证据吗）？
3、LightBluedye灵敏度如何？能比得过EB吗？
2、苏丹三 脂肪 橙红
3、苏丹四 脂肪 红
4、双缩脲 蛋白质 紫
5、龙胆紫 染色质 紫
6、碘 淀粉 蓝
7、健那绿 线粒体 绿
8、甲基绿 DNA 绿
9、吡罗红 RNA 红
10、溴麝香草酚蓝 CO2 由蓝变绿再变黄
11、重铬酸钾 酒精 酸性条件下由橙色变成灰绿
12、醋酸洋红（龙胆紫、改良苯酚品红） 染色质 红
13、台盼蓝 检验活死细胞 死细胞会被染成蓝色（不常用）
