
GradeMolecular Biology Grade
Specification
Appearance | White powder |
Mol. Formula | Not applicable. A polysaccharide complex. |
Mol. Weight | Not Applicable. |
Assay | Min | Max | Units |
Moisture content (≤ 10) | <10 | % | |
Sulfate content (≤ 0.15) | <0.15 | % | |
Gel Strength, 1.0% (≥ 1200) | 1340 | 2910 | g/cm2 |
EEO | 0.12 | -mr | |
Gelling Temperature | 36.0 | 37.0 | ˚C |
Melting Temperature | ≤90 | ˚C | |
Appearance | PASS | ||
RNase / DNase | Not Detected |
Ordering Information
Catalog No | Description | Pack Size |
PC0701-100g | Agarose (Molecular Biology Grade) | 100g |
PC0701-500g | Agarose (Molecular Biology Grade) | 500g |
PC0701-1kg | Agarose (Molecular Biology Grade) | 1kg |
DownloadMSDS
Agarose (Molecular Biology Grade)
PublicationThis Product Has Been Used In: Alshaibani et al. (2020) An improved procedure for the isolation of Ribonucleic acid from methicillin-resistant Staphylococcus aureus, Novel Research in Microbiology Journal, 4(2):704-713.Erkihun A & Kausalya R (2020) MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia, International Journal of Microbiology, Article ID 8853582.Lee Q et al. (2020) Murine and Related Chapparvoviruses are Nephro-tropic and Produce Novel Accessory Proteins in Infected Kidneys, PLOS Pathogens, 16(1): e1008262.Thongkao, K. & Sudjaroen, Y. (2020) Screening of antibiotic resistance genes in pathogenic bacteria isolated from tiny freshwater shrimp (Macrobrachium lanchesteri) and “Kung Ten”, the uncooked Thai food,Journal of Advanced Veterinary and Animal Research, 7(1):83-91Wannapa, Settheetham-Ishida et al. (2020)Genetic Polymorphism of Glutathione S-transferase and Cervical Cancer Susceptibility in Northeastern Thailand, Asian Pacific Journal of Cancer Biology, 5(2): 35-41.Tung HJ et al. (2019)cDNA-SSR Markers for Molecular Epidemiology of Ganoderma boninense,. Journal of Oil Palm Research, 31(2):220-237.Weiming, Su J. et al. (2019) Depth-sensitive Raman spectroscopy for skin wound evaluation in rodents, . Biomedical Optics Express, 10(12):6114-6128.Bo, E., Wang, L., Wu, X., Luo, Y., Chen, S., Chen, S., Liang, H., Ni, G., Yu, X., Liu, L. (2018) . Multiple aperture synthetic optical coherence tomography for biological tissue imaging, , Optics Express, Vol. 26, No. 2 (2018).Wong, C.Y. et al. (2018)Identification of Klebsiella pneumoniae in microwavable chicken curries using 16s rRNA analysis. International Food Research Journal, 25(2):620-625.Bo, E., Luo, Y., Chen, S., Liu, X., Wang, N., Gen, X. (2017).Depth-of-focus extension in optical coherence tomography via multiple aperture synthesis, Optica, Vol. 4, No. 7, 701-706 (2017).Chakdar, H., Pabbi, S. (2017).A comparative study reveals the higher resolution of RAPD over ARDRA for analyzing diversity of Nostoc strains,Springer,Vol. 7, No. 125. (2017).Chong,S.Y., Rao, P.V., Soon, J.M. (2017). Identification of Escherichia spp. strains in street-vended beverages and associated preparation surfaces using 16S rRNA analysis, International Food Research Journal, Vol. 4, No. 4, 1811-1818 CRivera, M.P., Cardenas, L.B. (2017). Species Delineation of Kaempferia galanga L. and Kaempferia pulchra L. in the Phillipines using psbA-trnH and petA-psbL-psbJ, . Biomedical Research, Vol. 16, No. 2., 84-93 (2017). Suleiman, E.A., Abdalla, W.G., Ahmed, A.H., Abdelgabar, M.A. (2017).Isolation and Molecular Characterization of Dermatophytes in Donkeys, , International Journal of Tropical Disease and Health, Vol. 26, No. 4, 1-8 (2017). Widyaningtyas, S.T., Soebandrio, F., Ibrahim, F., Bela, B. (2017). Design, synthesis, and functional testing of recombinant cell penetrating peptides. . Journal of Physics. (2017) Abu-Elsaad,N.M., Serrya, M.S., El-Karef, A.M., Ibrahim, T.M. (2016) The heat shock protein 90 inhibitor, 17-AAG, attenuates thioacetamide induced liver fibrosis in mice, Pharmacological Reports 68 (2016). 275–282 Aras, B., & Yerlikaya, A. (2016) Bortezomib and Etoposide Combinations Exert Synergistic Effects on the Human Prostate Cancer Cell Line PC-3. Oncology Letters. 11, p. 3179-3184.Luang-In,V., Deeseenthum, S. (2016) Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities. . LWT - Food Science and Technology.73. Pp592-601 Merghani, E.A.E., Elsanousi, S.M., Hamedelnil, Y.F., Gornas, N.A. (2016).Molecular Study of Bacterial Pathogens Isolated From Haemodialysis Patients, Sudan Journal of Science and Technology, Vol 12, No. 1, 1-10 (2016).Shamila-Syuhada, A.K., Rusul, G., Wan-Nadiah, W.A., Chuah, L. (2016).Prevalence and Antibiotics Resistance of Staphylococcus aureus Isolates Isolated from Raw Milk Obtained from Small-Scale Dairy Farms in Penang, Malaysia, Pakistan Veterinary Journal, Vol. 36, No. 1, 98-102 (2016). Vikneswaran, R., Eltayeb, N.E., Ramesh S., Yahya, R. (2016) New alicyclic thiosemicarbazone chelated zinc(II) antitumor complexes: Interactions with DNA/protein, nuclease activity and inhibition of topoisomerase-I. Polyhedron.105. Pp.89-95Wang, Z., Yang, T.D., S, A.S., Chang, M.S., Kershaw, S.V., Lo, P.K., Rogach, A.L. (2016) ) Aggregation-free DNA nanocage/Quantum Dot complexes based on electrostatic. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 495. Pp62-67.Watanapokasin, R., Imoto, M., Innajak, S., Kritsanawong, S. (2016).Antiproliferative and apoptosis induction of α-mangostin in T47D breast cancer cells, International Journal of Oncology, Vol. 48, No. 5 (2016). Ahmad, A.A.A.M., El-Obeid, E.A., Abushama, H. (2015). The Incidence of F8 Gene Mutation In Sudanese Patients at risk of both venous and arterial thrombosis under prolong use of Warfarin treatment, World Journal of Pharmaceutical Research, Vol. 3, No. 4, 27-34 (2017). Kepel, B., & Fatimawali, D. (2015) Analysis of 16SrNA Gene and Mercury Reduction Ability of Mercury Resistant Bacteria Isolated from Feces of Patient with Mercury Amalgam at Puskesmas Bahu in Manado. Jurnal Kedokteran Yarsi23(1), p.45-55.Malaitad, T., Poeaim, S., Eiamampai, K. (2015).Sex identification in barn swallows (Hirundorustica Linnaeus) by molecular technique, Journal of Agricultural Techonology, Vol. 11, No. 8, 2411-2418 (2015).Rahman, R.A., Sukri, N.M., Nazir, N.M., Aa’zamuddin, M., Radzi, A., Zulkifly, A.H., Ahmad, A.C., Hashi, A.A., Rahman, S.A., Sha’ban, M. (2015). The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering. Tissue and Cell 47 (4). Pp.420-430Saeed, I.K., et al (2015) First Report of Bovine Viral Diarrhea Virus Antigen from Pneumonic Cattle in Sudan Journal of Advanced Veterinary and Animal Research2(2), p.153-157. Vikneswaran, R., Syafiq, M.S., Eltayeb, N.E., Kamaruddin, M.N., Ramesh, S., Yahya, R. (2015)) A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 150. Pp.175-180.Cerasi, M., et al. (2014) The ZupT Transporter Plays an Important Role in Zinc Homeostasis and Contributes to Salmonella enterica Virulence. Metallomics. 6(4), p.845-853.Chan, S.N., et al. (2014) Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa. BioMed Research International. Hassanin, A., et al. (2014) Heparin Modulation on Hepatic Nitric Oxide Synthase in Experimental Steatohepatitis. Experimental and Theapeutic Medicine8, p. 1551-1558.Kaoud, H.A., Hussein, H.A., El-Dahshan, A.R., Kaliefa, H.S., Rohaim, M.A.(2014)Co-circulation of avian influenza viruses in commercial farms, backyards and live market birds in Egypt. International Journal of Veterinary Science and Medicine. 2(2). Pp.114-121Yuen, C. Liu, Q. (2014). Towards in vivo intradermal surface enhanced Raman scattering (SERS) measurements: silver coated microneedle based SERS probe, Journal of Biophotonics, Vol. 7, No. 9, 683-689 (2014).Nazree, N.E., et al. (2013) Lack of Association Between TPH2 Gene Polymorphisms with Major Depressive Disorder in Multiethnic Malaysia Population Asia-Pacific Psychiatryp.1-6. Rianthavorn, P., Chokedeemeeboon, C., Deekajorndech, T., Suphapeetiporn, K. (2013) Interleukin-10 promoter polymorphisms and expression in Thai children with juvenile systemic lupus erythematosus. Lupus. 22. Pp.721-726Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).
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二苯胺
甲基绿派洛宁
还有一种忘了。
假期没法联系老师呵呵。
这三种的作用都是什么。
产品主要应用:点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
菁染料是性能优良的荧光标记染料,摩尔吸光系数在荧光染料中是最高的。N-羟基琥珀酰亚胺酯是最常用的脂肪氨基标记试剂,广泛用于蛋白质、氨基酸多肽、抗体、核酸及其他生物分子的标记和检测。通过改变次甲基链的长度,可改变其荧光发射波长,每增加一个双键,按照Huoffman规则正好红移约100nm。
菁染料Cy3和Cy5已成为基因芯片的首选荧光标记物;另外,Cy5,Cy5.5和Cy7,Cy7.5的吸收在近红外区背景非常低,是荧光强度最高、最稳定的长波长染料,特别适合于活体小动物体内成像。但由于菁染料,尤其是不对称菁染料的合成副反应多,副产物极性相近,产物的分离提纯相当困难。菁染料特别是水溶性菁染料分子极性大,分离提纯越加困难。Lumiprobe供应脂溶性和水溶性菁染料。
相关产品:
产品分子结构可替代染料编号:AGF1371A
6-ROX-N-羟基琥珀酰亚胺酯
ROXNHSester,pure6-isomer[img]/KindEditor_4.0.1/attached/image/20130704/20130704102819_7906.jpg[/img]AlexaFluor568编号:AGF1326A
Cy3-N-羟基琥珀酰亚胺酯
Cy3NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103024_8531.jpg[/img]AlexaFluor546NHSester
DyLight549NHSester
编号:AGF1330A
Cy3.5-N-羟基琥珀酰亚胺酯
Cy3.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103242_2437.jpg[/img]AlexaFluor594NHSester
DyLight594NHSester
编号:AGF1338A
Cy5-N-羟基琥珀酰亚胺酯
Cy5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103422_4468.jpg[/img]AlexaFluor647NHSester
DyLight649NHSester
编号:AGF1345A
Cy5.5-N-羟基琥珀酰亚胺酯
Cy5.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103557_5875.jpg[/img]AlexaFluor680NHSester
DyLight680NHSester
编号:AGF1349A
Cy7-N-羟基琥珀酰亚胺酯
Cy7NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103713_8843.jpg[/img]编号:AGF1356A
Cy7.5-N-羟基琥珀酰亚胺酯
Cy7.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103756_5406.jpg[/img]编号:AGF1374A
磺酸基-Cy3-N-羟基琥珀酰亚胺酯
Sulfo-Cy3NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103830_1656.jpg[/img]AlexaFluor546
DyLight549
编号:AGF1377A
磺酸基-Cy5-N-羟基琥珀酰亚胺酯
Sulfo-Cy5NHSester[img]/KindEditor_4.0.1/attached/image/20130705/20130705095752_6656.jpg[/img]AlexaFluor647
DyLight649
编号:AGF1379A
磺酸基-Cy7-N-羟基琥珀酰亚胺酯
Sulfo-Cy7NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704104047_6968.jpg[/img]相似系列产品:
羰基活性荧光染料
氨基类染料
巯基反应性染料
羧酸类染料
2、苏丹三 脂肪 橙红
3、苏丹四 脂肪 红
4、双缩脲 蛋白质 紫
5、龙胆紫 染色质 紫
6、碘 淀粉 蓝
7、健那绿 线粒体 绿
8、甲基绿 DNA 绿
9、吡罗红 RNA 红
10、溴麝香草酚蓝 CO2 由蓝变绿再变黄
11、重铬酸钾 酒精 酸性条件下由橙色变成灰绿
12、醋酸洋红(龙胆紫、改良苯酚品红) 染色质 红
13、台盼蓝 检验活死细胞 死细胞会被染成蓝色(不常用)
分子生物学实验的染料主要涉及到核酸染料和蛋白质染料.核酸染料主要有EB(溴化乙锭,高致癌性),goldview,sybr green(实时定量PCR时常用染料).这些染料可以和核酸双链分子特异性结合发出强荧光而被检测到.蛋白质染料最常用的是考马斯亮蓝 R-250,硝酸银.其中硝酸银有时也用于核酸染色.
染料分为天然染料和人工染料两种。天然染料有胭脂虫红、地衣素、石蕊和苏木素等,它们多从植物体中提取得到,其成分复杂,有些至今还未搞清楚。目前主要采用人工染料,也称煤焦油染料,多从煤焦油中提取获得,是苯的衍生物。多数染料为带色的有机酸或碱类,难溶于水,而易溶于有机溶剂中。为使它们易溶于水,通常制成盐类。
染料可按其电离后染料离子所带电荷的性质,分为酸性染料、碱性染料、中性(复合)染料和单纯染料四大类。 标本干燥后即进行固定,固定的目的有三个:
1)杀死微生物,固定细胞结构。
2)保证菌体能更牢的粘附在载玻片上,防止标本被水冲洗掉。
3)改变染料对细胞的通透性,因为死的原生质比活的原生质易于染色。
前一阵子实验室开始使用一种新的核酸染料-goldview,取代原先用的溴化乙锭-EB.goldview现在是由赛百盛出售.以下摘自官方网站:
GoldViewTM核酸染料——使用说明
概述
GoldViewTM是一种可代替溴化乙锭(EB)的新型核酸染料,采用琼脂糖电泳检测DNA时,GoldViewTM与核酸结合后能产生很强的荧光信号,其灵敏度与EB相当,使用方法与之完全相同。在紫外透射光下双链DNA呈现绿色荧光,而单链DNA呈红色荧光。GoldView不仅能染DNA,也可用于染RNA。
通过Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠睾丸精母细胞染色体畸变试验,致突变性结果均为阴性;而溴化乙锭(EB)是一种强致癌剂。因此用GoldviewTM代替EB不失为一种明智的选择。
使用方法
1.将100ml琼脂糖凝胶溶液(浓度一般为0.8%~2%)在微波炉中融化。
2.加入5µlGoldView,轻轻摇匀,避免产生气泡。
3.冷却至不烫手时倒胶,待琼脂糖凝胶完全凝固后上样电泳。
4.电泳完毕在紫外灯下观察。若使用数码相机照像记录,则关闭相机的闪光灯,放在自动档即可;若使用凝胶成像系统照相,通过调节光圈、曝光时间,选择合适的滤光片,可得到成像清晰、背景较低的照片。
注意事项
1.胶厚度不宜超过0.5cm,胶太厚会影响检测的灵敏度。
2.加入GoldView的琼脂糖凝胶反复融化可能会对核酸检测的灵敏度产生一定影响,但不明显。
3.通过凝胶电泳回收DNA片段时,建议使用GoldView染色,在自然光下切割DNA条带,避免紫外线与EB对目的DNA产生的损伤,可明显提高克隆、转化、转录等分子生物学下游操作的效率。
4.虽然未发现GoldView有致癌作用,但对皮肤、眼睛会有一定的刺激,操作时应戴上手套。
电泳结果显示GV灵敏度与EB相当
问题1.Goldview到底是不是丫啶橙?
2.赛百盛不公布其成分的原因是害怕其为丫啶橙还是出于技术保密?
产品主要应用:点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
氨基类染料是包含自由氨基的活性染料,染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如:氨基与EDC-活化的羧基结合。
相关产品如下:
中文名英文名产品编号分子结构Cy7.5胺Cy7.5amineAGF1350A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110804_5250.jpg[/img]Cy5胺Cy5amineAGF1332A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110715_7750.jpg[/img]相似系列产品:
抗体、核酸、蛋白质等生物分子标记染料
羰基活性荧光染料
巯基反应性染料
羧酸类染料
请各位大侠给予帮助!!
谢谢!!
健那绿——高中唯一一个活体染色剂。染线粒体的,染成蓝绿色

