ORDERINGINFORMATION
CatalogNo.:43056
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Phosphataseandtensinhomolog(PTEN)wasidentifiedasatumorsuppressorthatismutatedinmanycancers.PTENisaphosphatidylinositol-3,4,5-trisphosphate3-phosphatase.Itcontainsatensin-likedomainaswellasacatalyticdomainsimilartothedualspecificityproteintyrosinephosphatases.Unlikemostproteintyrosinephosphatases,PTENpreferentiallydephosphorylatesphosphoinositidesubstrates.Itnegativelyregulatesintracellularlevelsofphosphatidylinositol-3,4,5-trisphosphateincellsandfunctionsasatumorsuppressorbynegativelyregulatingAkt/PKBsignalingpathway.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine380/threonine382/threonine383derivedfromhumanPTENandconjugatedtoKLH.
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratPTENonlywhenphosphorylatedatserine380andthreonine382/383.
Accessionno.:P60484,NP_000305.3
APPLICATIONS
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof
~54kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
Immunofluorescence:useatdilutionof1:100-1:200.
DetectionofPTEN(phospho-Ser380/Thr382/Thr383)inextractsofHeLacellstreatedwithIFNαorcalfintestinalphosphatase.
DetectionofPTEN(phospho-Ser380/Thr382/Thr383)inparaffin-embeddedhumanbreastcarcinomatissue.
DetectionofPTEN(phospho-Ser380/Thr382/Thr383)inmethanol-fixedMEFcells.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n.
同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
网络
同型;同种型;模式标本
双语例句
Study on the Action of Decreasing level of Isotype Cysteine for High Blood Viscosity
降低同型半胱氨酸水平对高黏血症作用的研究
比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
选用和特异性抗体同种亚型的，同样荧光素标记的抗体，采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合，那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚，是不需要同型对照的。另外，在多色同染的情况下，也不采用同型对照，而是采用FMO（fluorensence minus one）对照。
另外，对于同型对照的使用，也有很多反对意见。就算是同型抗体，也可能会有不同的亲和力，并不一定就能真实反应非特异性的结合。
