
HuntingtinPolyclonalAntibody
CatalogNo.:AB0080-200
Price/Unit:EUR320.00
Source:Goat
Quantity/unitsize:600µg
Description:HuntingtinisaproteinlinkedtoHuntington"sdisease,aneurodegenerativedisordercharacterizedbylossofstriatalneurons.Alternativenames:HD,HTTandIT15antibody.
Form:Polyclonalantibodysuppliedasa200µl(3mg/ml)aliquotinPBS,20%glyceroland0.05%sodiumazide.Thisantibodyisepitope-affinitypurifiedfromgoatantiserum.
Immunogen:Purifiedrecombinantpeptidederivedfromwithinresidues85to200aaofhumanHTTproducedinE.coli.
Specificity:Reactshuntingtinprotein,aminoacids85-200.Theantibodyrecognizeswildtypeandmutanthuntingtin.
Reactivity:Reactsagainsthuman,ratandmouseproteins.
Usage:Westernblot 1:500-1:2,000Immunofluorescence 1:500-1:2,000Immunohistochemistry(paraffin) 1:500-1:2,000Immunohistochemistry(frozen) 1:500-1:2,000
Reactivitychart
Sample | WesternBlot | Immuno-fluorescence | Immuno-Histochemistry(p) | Immuno-Histochemistry(f) | human | +++ | +++ | +++ | +++ | rat | +++ | +++ | +++ | +++ | mouse | +++ | +++ | +++ | +++ | canine | +++ | +++ | +++ | +++ | monkey | +++ | +++ | +++ | +++ |
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Storage:Storeat-20Cforlongtermstorage.Storeat2-8Cforuptoonemonth.
Specialinstructions:Avoidfreeze/thawcycles.

References
1.MiniarikovaJ,ZimmerV,MartierR,etal.GeneTher.2017Oct.PMID:287712342.MeunierC,MerienneN,JolléC,etal.Glia.2016Jul21.PMID:274424863.DéglonN,MerienneN,Genomeeditingforthetreatmentofhuntington"sdisease,EP2982758A1Patent,2014Forresearchuseonly,notfordiagnosticuse
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n.
同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
网络
同型;同种型;模式标本
双语例句
Study on the Action of Decreasing level of Isotype Cysteine for High Blood Viscosity
降低同型半胱氨酸水平对高黏血症作用的研究
比如你用anti-MHC-II,mouse IgG1-FITC,那同型就用非特异性的mouse IgG1-FITC,同样浓度
选用和特异性抗体同种亚型的,同样荧光素标记的抗体,采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合,那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚,是不需要同型对照的。另外,在多色同染的情况下,也不采用同型对照,而是采用FMO(fluorensence minus one)对照。
另外,对于同型对照的使用,也有很多反对意见。就算是同型抗体,也可能会有不同的亲和力,并不一定就能真实反应非特异性的结合。