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Hito Luxol Fast Blue - PAS OptimStain™ Kitis designed based on Luxol fast blue and Periodic acid-Schiff (PAS) method. This kit is made in a ready-to-use format and offers high quality, rapid staining (can be finished in one hour) of myelin/myelinated axons on paraffin sections or frozen sections. It has been proven to be extremely reliable and sensitive to demonstrate the morphological details of myelin fibers.Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. It is essential for the proper functioning of the nervous system. Demyelination is the loss of the myelin sheath insulating the nerves. This impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Luxol Hito Luxol Fast Blue - PAS OptimStain™ Kitallows sensitive localization and visualization of the myelin fibers. PAS counterstain provides color contrasts which show morphological features that are particularly significant in the tissue, thus offers a fast and reliable way to determine the extent of demyelination.Hito Luxol Fast Blue - PAS OptimStain™ Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
Kit Contents Standard Kit Small Kit Trial Kit Solution-1 (LFB Solution 10xConcentrate - for Trail Kit 2xConcentrate) 125 ml 60 ml 10 ml Solution-2 (Li2CO3 Solution 5xConcentrate - for Trail Kit 2xConcentrate) 250 ml 120 ml 10 ml Solution-3 (Periodic Acid Solution) 125 ml 60 ml 10 ml Solution-4 (Modified Schiffs Solution) 125 ml 60 ml 10 ml Solution-5 (Hematoxylin Solution) 30 ml 15 ml 7 ml Solution-6 125 ml 60 ml 10 ml Dropper Bottle 1 1 1 Staining Jars 6 6 5 User Manual and MSDS 1 1 1 Before using Hito Luxol Fast Blue - PAS OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat or Microtome and light microscopeParaffin embedding equipment(or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0104)Dry ice, isopentane, O.C.T. compound (for frozen sections), ethanol, xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized waterSlides and coverslipsStaining jars for slides washResinous mounting medium
Hito Luxol Fast Blue - PAS OptimStain™ Kit Manual and MSDS- Additional Information
Additional Information
SKU HTKNS1228-tr - Reviews
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n.
同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
网络
同型;同种型;模式标本
双语例句
Study on the Action of Decreasing level of Isotype Cysteine for High Blood Viscosity
降低同型半胱氨酸水平对高黏血症作用的研究
选用和特异性抗体同种亚型的，同样荧光素标记的抗体，采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合，那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚，是不需要同型对照的。另外，在多色同染的情况下，也不采用同型对照，而是采用FMO（fluorensence minus one）对照。
另外，对于同型对照的使用，也有很多反对意见。就算是同型抗体，也可能会有不同的亲和力，并不一定就能真实反应非特异性的结合。
手头上，有eBioscience家的同型抗体，为PE标记（mouseIgG1,k），说明书写使用为2.5ug/test。
按照说明书的浓度加入后结果发现，同型对照抗体组比实验组和阴性对照组（不表达CD80）荧光强度要高，很明确我的同型对照加入的剂量多了，但正确的加法是多少？按照质量算吗？请高手指教，非常感谢！！
