
Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
FIGF; VEGF-D; C-Fos Induced Growth Factor
- Product No.PAA146Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- ImmunogenEPA146Mu61-Eukaryotic Vascular Endothelial Growth Factor D (VEGFD)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.If the antibody is used in flow cytometry, please check FCM antibodies.
- Downloadn/a
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 97 For more details, please contact local distributors!US$ 227 For more details, please contact local distributors!US$ 324 For more details, please contact local distributors!US$ 810 For more details, please contact local distributors!US$ 3240 For more details, please contact local distributors!
SPECIFITY of the Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
The antibody isa rabbit polyclonal antibody raised against VEGFD. It has been selected for its ability to recognize VEGFD in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
Western blotting: 0.5-2µg/mL;1:250-1000Immunohistochemistry: 5-20µg/mL;1:25-100Immunocytochemistry: 5-20µg/mL;1:25-100Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Related products
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA146Mu01 | Recombinant Vascular Endothelial Growth Factor D (VEGFD) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA146Mu61 | Eukaryotic Vascular Endothelial Growth Factor D (VEGFD) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA146Mu02 | Recombinant Vascular Endothelial Growth Factor D (VEGFD) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA146Mu01 | Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD) | WB; IHC; ICC; IP. |
PAA146Mu02 | Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD) | WB; IHC; ICC; IP. |
SEA146Mu | ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA146Mu | Mini Samples ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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所以免疫组化在组织定位和定性上比较准确,但是在定量上不够准确。
western blot也是利用抗体和抗原之间的结合具有高度的特异性。经过聚丙烯酰胺凝胶电泳分离的蛋白质样品,转移到固相载体上,固相载体以非共价键形式吸附蛋白质,且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原,与对应的抗体起免疫反应,再与酶或同位素标记的第二抗体起反应。
western blot因为有内参标定,所以在定量上要更加准确。但是在组织定位上不如免疫组化
当然跟物种有关。
以上信息来自。
高保真生物技术有限公司~实验整体外包服务商~
因为内参有好几种,分子量差别也比较大,更换分子量与检测蛋白差距更大的内参就可以避免这个问题
另外也可以先用一抗孵育显色和检测,再用Strip缓冲液洗掉膜上的抗体,重新进行内参的抗体孵育显色检测。这样也可以将检测蛋白和内参显色在同一张膜上

