
Genome Amplifier (WGA)
- Fast and uniform amplification across entire genome within 90 min.
- Multiple Displacement Amplification by Phi29 DNA polymerase
- Whole genome amplification from as little as 10 ng of template DNA.
- More than 10 ㎍ of DNA products in a reaction
Products
Cat.No. | Product | Size |
---|---|---|
HGA001-50 | HelixAmp™ Genome Amplifier (WGA) | 50 rxns |
HGA001-100 | HelixAmp™ Genome Amplifier (WGA) | 100 rxns |
- Description
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HelixAmp™ Genome Amplifier is a whole genome amplification kit suitable to amplify the whole genome from very little amount of genomic DNA sample. The whole genome amplification using HelixAmp™ Genome Amplifier is performed by Phi29 DNA polymerase with an isothermal MDA (multiple displacement amplification) based mode. Because of the strand displacement activity, strong 3’→5’ exonuclease activity and extreme processivity, Phi29 DNA polymerase could produce DNA up to 100 kb long with high fidelity. About 1,000 fold amplified DNA can be obtained from 10 ng genomic DNA within 90 min using this kit. The amplified genome can be directly applied, without further purification, to the downstream genetic analysis works including PCR, genotyping, and library constrictions.
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既说像坐标画曲线曲线应纵轴该荧光强度吧轴数值变化代表荧光强度变化荧光强度变化代表钙离浓度变化 我觉直接用荧光强度值变化代替钙离浓度变化研究意义该钙离浓度变化吧
非要荧光强度值换算钙离浓度需要做列工作
1 取标准钙离浓度染色用共聚焦显微镜测荧光强度
2 标准品需要同像环境主要曝光间相同激光波及能量
3 标准品要用同浓度荧光染料
才能根据标准荧光强度关系推算品钙离浓度
1.怎么看两个反应-即目标基因和内参基因是否具有相同的扩增效率?
2.每次PCR反应的扩增情况都有差异,多次反应的扩增效率岂不是都不一样了吗?
3.如何设计才能让相对定量更可行?
由于对定量PCR的接触不多,希望各位高手多多帮助!谢谢!
什么是纯荧光校正,多长时间校正一次:
纯荧光校正是测定各种纯荧光染料标准品的波长和信号强度,通俗地说是让仪器“认识”各种荧光染料。软件收集并储存各种纯荧光染料标准品的荧光信息。以后每次定量实验运行过程中,SDS软件收集样品的原始光谱信号,并将此原始光谱与纯荧光文件中的数据进行比较,精确扣除不同染料的信号重叠部分,从而确定样品中的荧光染料种类和信号强度。
推荐每半年进行一次纯荧光校正。在运行光谱校正之前,请先进行背景校正和ROI校正。

