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biopioneerinc/96 well Genomic DNA miniprep kit - Animal Tissue, 5 x 96 well plates/1/GDMIP-96-1
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biopioneerinc/96 well Genomic DNA miniprep kit - Animal Tissue, 5 x 96 well plates/1/GDMIP-96-1
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biopioneerinc
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GDMIP-96-1
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4000-520-616

BP-10 96-Well Plate Genomic DNA Isolation Kit (For Animal)

Kit Contents:

Components

GDMIP-96-1,5-plates

GDMIP-96-2,10-plates

ACLSolution

PBSSolution

AB Solution

ProteinaseK

WashSolution

ElutionBuffer

BP-10 96-WellPlate

Deep WellCollection Plate

96-well storageplate

Sealingfilm

Protocol

200 ml

200 ml

200 ml

200 mg

4x48 ml

50 ml

5

10

5

25

1

2x200 ml

2x200 ml

2x200 ml

400 mg

8x48ml

100 ml

10

20

10

50

1

  • ACL Solution may form a precipitate upon storage. If necessary,dissolve the precipitate by warming the solution at 37ºC.
  • Before use, add 2ml of sterilized water to the tube containing 40 mg ofproteinase K, keep at -20 oC for long term.
  • Before use, add 200 ml of 100% ethanol to 50ml Wash Solution.
  • Elution Buffer is 2.0 mM Tris-HCl pH 8.0~8.5. Although TE buffer pH 8.0or water can be used, yield is generally 10% lower.

Storage: With the exception of theProteinase K, the kit may be stored at room temperature. The proteinase Kshould be stored at 4ºC for short term. The kit is stable for 18 months at roomtemperature. For longer storage, keep all contents cold.

Note: Thepurification method is based on centrifugation. There is a minimum heightrequirement of 75mm for apparatus to hold the assembly -- filter plate and deepwell plate.

Principle:

This kit is designed for fast isolation of genomicDNA from animal tissues. The kit contains a membrane embedded in BP-10 96-WellPlate for binding genomic DNA each well. Nucleotides, proteins, salts, andother impurities do not bind to the Column. Purified genomic DNA can be appliedin most molecular biology experiments including restriction digestion, PCR,Southern-blotting etc.

Applications:

Genomic DNA purificationfrom different animal tissues.

Features:

  • Preparation of high quality genomic DNA from variable sources.
  • Rapid and economical.
  • High yields
  • No phenol / chloroform extraction , no ethanol precipitation

Procedure for Isolation of Genomic DNA from Variable Sources.

For Animal Tissue

  1. Cut up to 30 mg tissue and place in Deep Well Collection Plate.
  2. Add 300 ul of ACL Solution (Animal Cell Lysis Solution) to Deep WellCollection Plate and 20ul proteinase K, then seal.
  3. Incubate at 55oC until the tissue is completely lysed (usually 1-3 hours). Occasionallyvortex. Incubation in shaking water bath can reduce lysis time.
  4. Cool to room temperature. Vortex for 20 seconds and Centrifuge 8,000rpmfor 5 minutes.
  5. Pipette 300ul of supernatant into a BP-10 96-Well Plate (if pellet notvisible, repeat previous step) and add 300ul of AB Solution, Seal, Mix by occasionallyinverting Plate, and keep for 2 minutes.
  6. Place a 96-Well Plate on thetop of a fresh Deep Well Collection Plate. Centrifuge at 6,000 rpm for 5 minutes witha rotor for microtiter plates.
  7. Discard flow-through. Add500 ul Wash Solution to each well of 96-Well Plate and spin at 6,000 rpm for 5minutes. Discard flow-through and place BP-10 96-Well Plate back to the sameDeep Well Collection Plate.
  8. Add 500 ul Wash Solution toeach well of the BP-10 96-Well Plate, spin at 6,000 rpm for 5 minutes. Discardflow-through and spin once more for 5 minutes to remove residue of WashSolution.
  9. Transfer the BP-10 96-WellPlate to a 96-well storage plate. Add 30-50 ul Elution Buffer to the BP-1096-well plate; incubate at 50 oC for 2minutes. Centrifuge at 6,000 rpm for 5 minutes.
  10. Measure DNA quantity by UV absorption at A260 (1.0 OD unit isequivalent of 50 ug). Assess genomic DNA quality by an analytical 0.7% agarosegel. Isolated genomic DNA should not contain RNA. Its length should be over 50kb.

For Rodent Tail

  1. Place Deep Well Collection Plate in dry ice.
  2. Cut 0.5cm to 1cm from end of tails and place in Deep Well CollectionPlate.
  3. Add 300 ul of ACL Solution (Animal Cell Lysis Solution) to Deep WellCollection Plate and 20ul proteinase K, then seal.
  4. Incubate at 55 oC overnight with rocking or for several hours with occasional mildvortexing every 15 minutes.
  5. Cool to room temperature. Vortex20 seconds and Centrifuge 8,000rpm for 5 minutes.
  6. Pipette 300ul of supernatant into an BP-10 96-Well Plate (if pellet notvisible, repeat previous step) and add 300ul AB Solution, Seal. Mix byoccasionally inverting Plate,and keep for 2 minutes.
  7. Add 500 ul Wash Solution toeach well of the BP-10 96-Well Plate, spin at 6,000 rpm for 5 minutes. Discardflow-through.
  8. Repeat washing step 7
  9. Discard flow- through and spinonce more for 5 minutes to remove residue of Wash Solution.
  10. Transfer the BP-10 96-WellPlate to a 96-well storage plate. Add 30-50 ul Elution Buffer to the BP-1096-well plate; incubate at 50 oC for 2minutes. Centrifuge at 6,000 rpm for 5 minutes.
  11. Genomic DNAis ready for use or kept at –20 oC.

For Cultured Animal Cell (NEW!)

  1. Centrifuge the appropriate number of cells(>5x106) for 5minutes at 2,000rpm.
  2. Resuspend pellet in 500 ul of PBS Solution.
  3. Wash the cells 2 times with PBS.
  4. Resuspend pellet in 300ul of ACL solution buffer.
  5. Add 20ul of proteinase K.
  6. Incubate at 55oC for 10 minutes.
  7. Cool to room temperature.Vortex for 20 seconds and Centrifuge 8,000rpmfor 5 minutes.
  8. Pipette 200ul of supernatant into an BP-10 96-Well Plate (if pellet notvisible, repeat previous step) and add 200ul AB Solution. Mix by occasionallyinverting Plate, and keep for 2 minutes.
  9. Centrifuge 6,000rpm for 5 minutes and discard theflow-through.
  10. Add 500 ul of Wash Solution, and spin at 6,000 rpm for 5 minutes.
  11. Repeat washing step 10
  12. Discard flow- through and spin once more for 5 minutes to removeresidue of Wash Solution.
  13. Transfer the BP-10 96-WellPlate to a 96-well storage plate. Add 30-50 ul Elution Buffer to the BP-1096-well plate; incubate at 50 oC for 2minutes. Centrifuge at 6,000 rpm for 5 minutes.
  14. Genomic DNAis ready for use or kept at – 20 oC.
  15. Measure DNA quantity by UV absorption at A260 (1.0 OD unit isequivalent of 50 ug). Assess genomic DNA quality by an analytical 0.7% agarosegel. Isolated genomic DNA should not contain RNA. Its length should be over 50kb.

FromParaffin Tissue

  1. Excise 25~30mgparaffin tissue with a clean, sharp scalpel.And transfer to a DeepWell Collection Plate.
  2. Add 1.2ml xylene (self-prepared by user) to Deep Well Collection Plate, Seal, and thenvortex for 3minutes.Xylene is used to remove paraffin.
  3. Centrifuge at 12,000rpm for 5 minute at room temperature.
  4. Remove the supernatant completely. Keep the pellet.
  5. Add 1.2ml 100% of ethanol to Deep Well Collection Plate, Seal, Gently vortex for 1min. Incubate at room temperature for 1 minute.
  6. Centrifuge at 8,000rpm for 5 minute at room temperature. Discard supernatantcompletely.
  7. Repeat washing steponce from step4 to 6.
  8. Incubateat 37 oC for10-15 minutes to remove residual ethanol.
  9. Resuspend the sample in 200ul TE buffer, and continue immediately with Step10.
  10. Add 300 ul of ACL Solution(Animal Cell Lysis Solution) to Deep Well Collection Plate and add 20ulproteinase K, then seal it.
  11. Incubate at 55oC until the tissue is completely lysed (usually1-3 hours). Occasionally vortex.
  12. Cool to room temperature.Vortex for 20 seconds and Centrifuge 12000rpm for 5 minutes.
  13. Pipette 300ul of supernatantinto an BP-10 96-Well Plate (if pellet not visible, repeat previous step) andadd 300ul of AB Solution, Seal. Mix by occasionally inverting Plate, andkeep for 2 minutes.
  14. Centrifuge 6000rpm for 2 minutes and discard theflow-through.
  15. Add 500 ul of Wash Solution,and spin at 6,000 rpm for 5 minutes.
  16. Repeat washing step 15.
  17. Discard flow-through. Spinat 6,000 rpm for 5 minutes to remove residual amount of Wash Solution.
  18. Place the BP-10 96-WellPlate to 96-well storage plate. Add 30-50 ul Elution Buffer into the BP-10 96-WellPlate. Incubate the tube at 37 or 50 oC for 2minutes. Incubate at 37 or 50 oC couldincrease recovery yield.
  19. Spin at 8,000 rpm for 5minutes to elute DNA from the column.
  20. Measure DNAquantity by UV absorption at A260 (1.0 OD unit is equivalent of 50ug). Assessgenomic DNA quality by an analytical 0.7%agarose gel. Isolated genomic DNA should not contain RNA. Its length should beover 50 kb.

Biopioneer Brand:

GDMIP-96-1 (96-well_genomic_kit-animal.doc, 64Kb) [Download]

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应用很广泛,因为比荧光定量PCR的操作简单方便,无需专业人员操作,仪器的性价比高,挺受食品企业的追捧,食药系统也用的不少,现在技术越来越成熟,以后的应用会越来越广
大家好,现在做LAMP结果颠倒了,阴性对照会出现LAMPladder条带,而加了基因组DNA的反而扩增不出来,有没有人遇见这种情况?最后如何解决了呢?
主要是该方法使用了Bst酶,双链的DNA有一个特性,就是在65度时双链之间的结合较为松散,称之为动态平衡状态,所以不需要预变性使双链解开,而Bst酶在65度时的活性是最高的,这样LAMP反应就能正常进行了
扩增一个鸭的基因,PCR仪Bio-Rad的mycycler系列,通过升级后可以做梯度(8个温度梯度)PCR。
近期老是出问题,梯度(47~53℃)扩增时共6个温度有较明亮的条带,确定恒温再次扩增,却无任何产物。
请教大家了,着急的很!
各位战友:

今天发现,我在核酸技术区开辟的“DIG/Biotin标记的Southern/Northern杂交专题讨论”4个月,今天回帖数破100,点击达1500多次。能和大家一起分享和交流,并且得到共同提高,心情很是愉快。

所以想借PCR技术区的宝地,新开辟“环介导等温扩增技术(Loop-MediatedIsothermalAmplification,LAMP)专题讨论”。我做LAMP一年多了,有些经验。而最近,和我谈起LAMP的朋友明显增多。所以在这方面大家有什么心得、体会、资料和实验困难,希望能一起交流讨论。谢谢支持!
欢迎讨论环介导的恒温扩增法(LAMP)
(1)严格分区操作,样品提取区、配液区、扩增区、检测区应相互独立,各区耗材及移液器应专用,实验前先用紫外线消毒以破坏残留的核酸。
(2)操作多份样品时,应先配制反应混合液并分装,减少操作,避免污染,同时增加反应的精确度。试剂的配置和分装应在装有紫外灯的超净工作台或负压工作台操作。
(3)移液器和吸头需专用。由于移液器极易受气溶胶或模板核酸的污染,移液器应尽可能使用可替换或可高压处理的,吸头尽可能使用带滤芯的吸头。
(4)防止操作人员污染,使用一次性手套,EP管与吸头应一次性使用,吸头不要长时间暴露于空气中,避免气溶胶的污染。
(5)加样或吸取模板核酸时要十分小心,吸样要缓慢,防止样品进入移液器内;吸样时尽量一次性完成,忌多次抽吸,以免交叉污染或产生气溶胶污染。
(6)EP管打开应先离心,将管壁及管盖上的液体甩至管底部。开管动作要轻,以防管内液体溅出。若不小心溅到手套或桌面上,应立刻更换手套并用稀酸擦拭桌面。
(7)模板核酸应密封保存,防止外溢及外来核酸的进入。
(8)设立适当的阴阳性对照及重复实验,既可验证LAMP反应的准确性,又可以协助判断扩增系统的可信性。
(9)扩增产物应妥善处理,尽量避免开盖检测,开盖极易产生气溶胶污染。本公司的试剂均采用闭管检测,有效防止污染发生。展开
有人知道交叉引物核酸恒温扩增(CPA)的扩增原理吗?希望高手不吝赐教,最好是图文并茂的那种,谢谢了先!
基因扩增技术(PCR)聚合酶链反应(polymerase chain reaction简称PCR)又称无细胞分子克隆系统或特异性DNA序列体外引物定向酶促扩增法,是基因扩增技术的一次重大革新。可将极微量的靶DNA特异地扩增上百万倍,从而大大提高对DNA分子的分析
欢迎加入环介导的恒温扩增法(LAMP)讨论群70918628
基因扩增技术(PCR)聚合酶链反应(polymerase chain reaction简称PCR)又称无细胞分子克隆系统或特异性DNA序列体外引物定向酶促扩增法,是基因扩增技术的一次重大革新。可将极微量的靶DNA特异地扩增上百万倍,
技术原理
同一温度下,首先通过M-MLV反转录酶产生靶标核酸(RNA)的一个双链DNA拷贝,然后利用T7RNA多聚酶从该DNA拷贝上产生多个(100~1000个)RNA拷贝;每一个RNA拷贝再从反转录开始进入下一个扩增循环;还可以加入荧光探针(分子信标),带有荧光标记的探针和这些RNA拷贝特异结合,产生荧光。该荧光信号可由实时荧光PCR仪实时捕获,直观反映扩增循环情况。

SAT检测技术的优势
→先进的恒温扩增技术
核酸扩增在一个温度下进行(42℃),无需热循环。
→领先的RNA检测技术
SAT技术直接以病原体特异性RNA为扩增靶标,以扩增产物RNA为检测靶标,在实际应用中更显优势意义。
→更高的检测灵敏度和准确性
SAT技术的扩增效率极高,15~30分钟即可将模板扩增109倍,检测灵敏度和准确性远高于其他核酸检测技术。扩增时间短,效率高。
→有效减少假阴性结果
SAT技术采用M-MLV逆转录酶和T7RNA多聚酶进行核酸扩增,相对于其它核酸扩增技术,反应抑制物更少,有效减少假阴性结果。
→有效的解决了扩增产物的污染问题
由于扩增产物为RNA,环境中极易降解,从而大幅度提高检测结果的可靠性。
→大大降低了核酸扩增实验室的要求
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