N2developmenttimesatdifferenttemperatures Determinedempirically;timesinhoursaregivenfromthefirstdivisionofthezygote. Forstaginglateranimals:L4"scanbeeasilyrecognizedandstagedtowithinafewhoursofdevelopment.BypickingL4"sandthenagingthem,afairlysynchronouspopulationofadultsofknownagecanbeobtained;thisisespeciallyusefulforbehaviouralassays,theresultsofwhichmaydependonage. L4hermaphorditesarerecognizedbyawhitecrescentshapeatthelocationofthedevelopingvulva,about2/3ofthewaytowardstheposterioroftheanimal,onit"s"side"asitliesontheplate.Thisstandsoutincontrasttothedarkeryellowgrayofthesurroundingbody.Overtime,theL4"sgetfatterrelativetotheirlength,adarkspotappearsinthemiddleofthewhitecrescent,andfinallythewhitecrescentcollapsespriortotheL4molt.ThussubstagesofL4arerecognizable. L4malesarerecognizedbyatailthatisjustbeginningtoacquireaspadeshape,buthasnotachievedthewelldefinedsharplycorneredspade-tailoftheadult.Sinceyoungmalesarebetteratmating,itisbesttosetupmatingswithL4males.eggslaid hatch L1molt L2molt L3molt L4molt