The NANOSENSORS uniqprobe combines the well-known features of the other NANOSENSORS AFM probe series such as high application versatility and compatibility with most commercial SPMs with the additional advantage of a strongly reduced dispersion of force constant and resonance frequency.
The unsurpassed uniformity of the mechanical characteristics of the uniqprobe series is particularly important for applications, where a large number of probes with known and near identical force constants or resonance frequencies are needed. The sensors of the uniqprobe series are especially adapted for molecular biology, biophysics and quantitative nano-mechanical studies.
NANOSENORS qp-BioT AFM probes are designed for contact mode or dynamic mode AFM imaging in air or liquid environment. These probes feature two different triangular cantilevers on one side of the support chip. The uniqprobe BioT type offers an alternative to silicon nitride probes, with the advantage of taller tips with smaller opening angles and reduced drift.
The probe offers unique features:
small dispersion of force constant and resonance frequency
typical tip height 7µm
typical tip radius of curvature smaller than 10nm
stress free cantilevers with considerably less bending
tip and cantilevers are made of a quartz-like material
reduced drift for applications in liquid environments
tip repositioning accuracy of better than ± 8 µm (in combination with Alignment Chip)
chemically inert
A chromium/gold layer of about 60nm is partially coating the cantilever on the detector side near its free end where the tip is situated. The main advantages of this partial metallic coating are considerably less cantilever bending and reduced drift for SPM measurements in liquid environments.
AFM Tip:
Circular symmetric
7 µm (5.5 - 8.5 µm)*
< 10="" nm="">(< 15="" nm="">
AFM Cantilevers:
Cantilever 1
Triangle
100 µm (95 - 105 µm)*
14 µm (13 - 15 µm)*
900 nm (870 - 930 nm)*
0.3 N/m (0.15 - 0.45 N/m)*
50 kHz (42 - 58 kHz)*
Cantilever 2
Triangle
200 µm (195 - 205 µm)*
28 µm (27 - 29 µm)*
900 nm (870 - 930 nm)*
0.08 N/m (0.06 - 0.12 N/m)*
20 kHz (16 - 24 kHz)*
* guaranteed range This product features alignment grooves on the back side of the holder chip.
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近日,中科院大连化学物理研究所杨凌团队探索出酶特异性探针底物的研发新策略,该团队采用计算机辅助筛选及酶催化位点局部改造的策略成功设计研发了首个细胞色素P450 1A1亚酶的高特异性荧光探针底物。相关研究成果以“A practical strategy to design and develop an isoform-specific fluorescent probe for a target enzyme: CYP1A1 as a c 查看更多>
AlleleID. It is designed to address the challenge of pathogen detection, species identification and taxa discrimination using TaqMan and molecular beacon assays. With ClustalW multiple sequence alignment at its core, AlleleID can help design universal or taxa/species specific primers and probes. All major qPCR assays including molecular beacons, TaqMan, FRET or SYBR Green are supported. It uses BLAST search to assure specificity. To assure assay success, AlleleID uses template folding to avoid locating primers in the secondary structures of the template.
With AlleleID, you can avoid gDNA by designing primers/probes across exon/exon boundaries. The intron exon regions are identified by interpreting GenBank annotations or by specifying them manually.
To get started quickly with AlleleID, please download and listen to the multi-media tutorial: http://www.PremierBiosoft.com/DATAFILES/AL100Tour.exe.
Then please download the demo version of the program from: http://www.premierbiosoft.com/DATAFILES/AlleleIDTour.exe