The new PointProbe®Plus (PPP) combines the well-known features of the proven PointProbe® series such as high application versatility and compatibility with most commercial SPMs with a more reproducible tip radius as well as a more defined tip shape. The minimized variation in tip shape provides more reproducible images.
The PPP-EFM probe is offered for electrostatic force microscopy. An overall metallic coating (PtIr5) on both sides of the cantilever increasing the electrical conductivity of the tip. The force constant of this type is specially tailored for the electrostatic force microscopy yielding very high force sensitivity while simultaneously enabling tapping mode and lift mode operation.
The probe offers unique features:
metallic conductivity of the tip
high mechanical Q-factor for high sensitivity
precise alignment of the cantilever position (within +/- 2 µm) when used with the Alignment Chip
compatible with PointProbe® Plus XY-Alignment Series
Please note:
Wear at the tip can occur if operating in contact-, friction- or force modulation mode.
Although this is possible, it is not recommended to use PtIr5 coated tips for electrical contacting in applications where it is necessary to conduct high current. The very thin layer of PtIr5 is unable to support much current.
The PtIr5 coating is an approximately 25 nm thick double layer of chromium and platinum iridium5 on both sides of the cantilever. The tip side coating enhances the conductivity of the tip and allows electrical contacts. The detector side coating enhances the reflectivity of the laser beam by a factor of about 2 and prevents light from interfering within the cantilever. The coating process is optimized for stress compensation and wear resistance. The bending of the cantilever due to stress is less than 3.5% of the cantilever length.
AFM Tip:
AFM Cantilever:
Beam
225 µm (215 - 235 µm)*
28 µm (20 - 35 µm)*
3 µm (2 - 4 µm)*
2.8 N/m (0.5 - 9.5 N/m)*
75 kHz (45 - 115 kHz)*
* guaranteed range This product features alignment grooves on the back side of the holder chip.
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AlleleID. It is designed to address the challenge of pathogen detection, species identification and taxa discrimination using TaqMan and molecular beacon assays. With ClustalW multiple sequence alignment at its core, AlleleID can help design universal or taxa/species specific primers and probes. All major qPCR assays including molecular beacons, TaqMan, FRET or SYBR Green are supported. It uses BLAST search to assure specificity. To assure assay success, AlleleID uses template folding to avoid locating primers in the secondary structures of the template.
With AlleleID, you can avoid gDNA by designing primers/probes across exon/exon boundaries. The intron exon regions are identified by interpreting GenBank annotations or by specifying them manually.
To get started quickly with AlleleID, please download and listen to the multi-media tutorial: http://www.PremierBiosoft.com/DATAFILES/AL100Tour.exe.
Then please download the demo version of the program from: http://www.premierbiosoft.com/DATAFILES/AlleleIDTour.exe