Applications:
- AffinitymatrixwithimmobilizedStreptavidinbyCNBractivationcouplingmethod.
- Thebasematrixisarigid,highlycross-linked beadedagarosewithhighchemicalstABIlity.
- Theimmobilizedstreptavidinbinds biotinandbiotinylatedsubstancesandcanbeusedforaffinitychromatography applications.
- Theinteractionbetweenstreptavidinandbiotinisverystrongand requiresdenaturatingconditionsforelution,whichmaydestroyboththeligandandthesample.
- Strptavidin-4Bisextremelyusefulforexploitingeitherthestronginteractionsofbiotinandstreptavidinorthesomewhatweakerinteractionsof2-iminobiotinandstreptavidin.
- Itcanbeusedinthepurificationofantigens,wherebiotinylated antibodiesareincubatedwithantigen.Thebiotinylatedantibody-antigen complexbindstoStreptavidinSeparoporefromwhichtheantigencanbeeluted.
Technicalspecifications
- Ligand:NativeStreptavidin(from Streptomycesavidinii)
- Matrix:agarosebeads,4%
- Particlesizerange:52-165µm
- Matrixactivation:Cyanogenbromide
- Liganddensity:2mgstreptavidin/mldrainedgel
- Bindingcapacity:>20µgbiotin/mldrainedgel
- Exclusionlimit:~2x107
- pHstability:3–10
- Bindingbuffer:50mMammoniumcarbonate,0.5MNaCl,pH10
- Elutionbuffer:50mMammoniumacetate,0.5MNaCl,pH4
- SuppliedassUSPensioninbufferedsalinesolution.
AdditionalInformation
| Product# | S-2913 |
|---|---|
| Appearance | LiquidSuspension |
| StorageTemp | 4°C |
