High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- Product No.HEA071Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.62-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.8pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 434 For more details, please contact local distributors!US$ 620 For more details, please contact local distributors!US$ 2790 For more details, please contact local distributors!US$ 5270 For more details, please contact local distributors!US$ 43400 For more details, please contact local distributors!
Specificity of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 1 Alpha (IL1a).No significant cross-reactivity or interference between High Sensitive Interleukin 1 Alpha (IL1a) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
Matrices listed below were spiked with certain level of recombinant High Sensitive Interleukin 1 Alpha (IL1a) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Interleukin 1 Alpha (IL1a) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-98 | 85 |
EDTA plasma(n=5) | 78-99 | 80 |
heparin plasma(n=5) | 81-97 | 89 |
Precision of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 1 Alpha (IL1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 1 Alpha (IL1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>10%>Inter-Assay: CV<12%>12%>
Linearity of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Interleukin 1 Alpha (IL1a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 85-96% | 86-96% | 79-101% | 99-105% |
EDTA plasma(n=5) | 91-99% | 90-97% | 99-105% | 97-104% |
heparin plasma(n=5) | 95-103% | 85-101% | 82-92% | 86-95% |
Stability of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
RPA071Ra02 | Recombinant Interleukin 1 Alpha (IL1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
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KSA071Ra01 | ELISA Kit DIY Materials for Interleukin 1 Alpha (IL1a) | Main materials for "Do It (ELISA Kit) Yourself". |
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1.标准曲线的标准品是否一定要梯度稀释,为什么?我试过非梯度稀释的,也可以达到线性R2=0.99.
2.我用了CurveExpert做标曲,自动搜索后发现有10种提供的方程,各种形式的,其中一个十分适合我的实验结果(LogisticModel),而其他的感觉又不适合,因为结果常常为负值。这又是为啥捏?
3.实验的酶标仪最大OD值可以测到4,如果我的测量结果在1.3,是否像其他人所说的>1了就不准确了。
4.利用夹心法进行定量分析是否一定要使用线性方程?
不好意思啊,一下问了这么多问题,最近做了一个月的ELISA,完全摸不清头脑啊。谢谢各位了
我现在用商品化的试剂盒进行夹心法ELISA测定某抗体的浓度,盒子给定了4个标准品,标准品抗体浓度为70000~1000单位,测定步骤中要求对样品进行1:1000稀释后测定。
但是有的样本按照1:1000稀释后,最终OD值大于70000浓度的标准品的OD值,然后使用ELISACALC软件进行四参数拟合,超过了标准曲线的范围,就算不出来这个样品的浓度,但是如果在1000-70000之间的OD值就可以算出相应浓度。请教大神们,接下来我是否可以对样品进行1:2000,1:4000浓度稀释,算出结果后再×2、×4,算作此样品的浓度呢?还是直接给一个>70000的定性结果?或者可以有能算出来的其他软件?
Ps试剂盒中提及Dilutionlinearity(稀释线性?)为141%,这个是什么意思呢?
多谢!!
1. 直接法测定抗原 2.间接法测定抗体 3.双抗体夹心法测定抗原4. 竞争法测定抗原 厚百生物专业提供各类生化实验试剂、仪器、耗材,提供技术服务,满足您的实验室常规采购需求。厚百,让您更省心!
请大家帮帮忙,第一步加完抗原后必须封闭吗