
Components | Bicinchoninic AcidProtein Assay Reagent A - 450 mlBicinchoninic Acid(BCA) Protein Assay Reagent B - 10 ml |
---|---|
Sensitivity | Detect down to 5 µg/mL with the enhanced protocol |
Linearity | Linear working range for BSA equals 20 to 2000 µg/mL |
Bicinchoninic(BCA) Acid Protein Assay Kitis a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard.
Bicinchoninic(BCA) Acid Protein Assayprovides accurate determination of protein concentration with most sample types encountered in protein research.Bicinchoninic(BCA) Acid Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
3′-Full RACE Core Set Ver.2.0D314 20 次 1,800 元
5′-Full RACE Kit D315 RT反应10次 PCR反应50 次 3,980 元
takara的做得出来不,请各位高手指教,谢谢了!
RACE是基于PCR技术基础上由已知的一段cDNA片段,通过往两端延伸扩增从而获得完整的3'端和5'端的方法
RACE技术的原理和操作方法:http://wenku.baidu.com/link?url=J-9lPSGmRLQriSfXhOqcYYFvJx7ktGIRMgU6k9vDQIywtdJ8deNg7Qi5t4CgtjxuhWUBvQO6_qsULiqUIFckEZty75jYNw5yz6rNqiIKCwm
我也想问一下。
步骤:1,RNA抽提(同RT)
2,逆转录,使用合成的oligo
3,p内参
4,使用根据已知序列设计的上游引物和提供下游引物做PCR,注意设计上游引物是退火温度与下游引物相差不大。
5,产物切胶回收,TA克隆测序
这个oligo和下游引物是我根据Takara和BD公司的manual自己设计的,经过我的使用效果很好。具体操作参照takara的3-racemanual。你先做3-race,了解熟悉下,成功后再做5-race。5-race要买试剂盒做,较为复杂!
请具体点,把联系方式和价位都说一下好吗?

