
- SynonymNoggin,NOG,SYM1,SYNS1
- SourceHuman Noggin, Fc Tag (NON-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Cys 232 (Accession # Q13253-1).Predicted N-terminus: Gln 28Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.2 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human Noggin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds
Authors: Ye J, et al.
Journal: Cell Res 2015
Application: Cell culture
Request for Full-text
- BackgroundNoggin is also known as NOG,SYM1, SYNS1 and is a secreted homodimeric glycoprotein whose scaffold contains a cystine-knot topology similar to that of BMPs.Secreted Noggin probably remains close to the cell surface due to its binding of heparincontaining proteoglycans.Noggin inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion. During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning. During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons Noggin also appears to maintain adult stem cell populations in vivo, for example, maintaining neural stem cells within the hippocampus.
- References
- (1)Valenzuela, D.M. et al. 1995, J. Neurosci. 15:6077.
- (2)Groppe, J. et al. 2002, Nature 420:636.
- (3)Itsykson, P. et al. 2005, Mol. Cell. Neurosci. 30:24.
- (4)Xu, R.H.et al. 2005, Nat. Methods 2:185.
- (5)Bonaguidi, M.A. et al. 2008, J. Neurosci. 28:9194.
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减数分裂也是真核生物细胞增殖的方式之一。
真核生物细胞增殖的方式有:有丝分裂、无丝分裂和减数分裂。
EdU是一种胸腺嘧啶核苷类似物,能够在细胞增殖时期代替T渗入正在复制的DNA分子,通过基于EdU与Apollo®荧光染料的特异性反应检测DNA复制活性,通过检测EdU标记便能准确地反映细胞的增殖情况。与BrdU检测方法相比,EdU检测方法更快速、更灵敏、更准确。EdU检测染料只有BrdU抗体大小的1/500,在细胞内很容易扩散,无需DNA变性(酸解、热解、酶解等)即可有效检测,可有效避免样品损伤,在细胞和组织水平能更准确地反映细胞增殖等现象。
比如用CD3、CD28刺激,除了这个还有什么方法吗?增殖!
and还想问问用CD3、CD28刺激是不是所有T细胞都能增殖?
本研究从新合成的十种核苷类似物中筛选具有抗肿瘤活性的化合物, 观察其抑制肿瘤细胞增殖、诱导细胞凋亡的作用,并探讨可能的作用机制。
做一个药物对细胞增殖和迁移的抑制的研究,已知药物对细胞增殖有影响,想要继续做迁移实验,怎么排除药物对细胞的抑制作用对细胞迁移产生的影响?谢谢大家!
首先,可以让学生设想细胞数目增加需要那些方面准备?过程会怎么样?这实际上是学生主体性的体现,有利于学生总体把握细胞增殖的变化规律。
然后,要让学生明白细胞内有好多变化,但我们的学习集中在染色体的变化规律来教学,重点一定要突出染色体这样的变化是为了遗传性状的稳定。复制、螺旋化、移到赤道面、着丝粒分裂、纺锤丝牵拉都是为了染色体结构和数目的稳定,这种动态变化完全可以设想出来,不需要去死记硬背。
最后,由于概念繁杂,一定要对概念进行辨析:染色体和染色质;纺锤体和纺锤丝;赤道面和细胞板。

