Overview
Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right or double-sided size selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek’s proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments and eliminate excess nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.
- No protocol change against major competitor
- Double-sided size selection
- DNA Clean-Up: PCR Clean-Up
- RNA Clean-Up: cDNA or RNA purification
- Manual or adaptable to most open-ended liquid handlers
- Significant cost savings
- 96- or 384-well formats
Protocols are available for the following automated platforms:
- Hamilton Microlab® STAR
- Hamilton Microlab® NIMBUS
- KingFisher™, BioSprint®, and MagMAX® 96
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream application | Cloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 15 µL or above |
| Starting material | DNA or RNA: PCR products, gDNA, cDNA |
| Starting amount | Scalable |
| DNA recovered | >90% recovery for DNA >100 bp |
| Processing mode | Automated; manual |
| Throughput | 96-384 samples per run |
| DNA binding technology | Magnetic beads |
| Storage | 2°C - 8°C |
| Special note | Size selection by varying beads ratio |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
M1378 Mag-Bind TotalPure NGS
QUICK GUIDE
M1378 Mag-Bind TotalPure NGS
SDS
M1378 SDS
SALES SHEET
REFERENCE
University of Oregon Evaluation of Mag-Bind TotalPure NGS for DNA Size Selection
APPLICATION NOTE
Automated DNA Cleanup for PCR and NGS Workflows: Mag-Bind® TotalPure NGS on Tecan Fluent® 780 Workstation
Product Data
Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-side size selection.
Figure 1. Electropherogram overlay of the double-sided size selection on sheared gDNA at 0.8x/0.7x ratio set using Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable kit from Company A following manufacturer’s recommended protocols. The DNA was eluted in 25 µL and analyzed on Agilent’s TapeStation® 2200.
Mag-Bind TotalPure NGS from Omega Bio-tek has excellent recovery of targeted DNA fragments.
Figure 2. 10 µL of 50 bp ladder was purified with Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A following manufacturer’s recommended protocols. The DNA was eluted in 20 µL and analyzed on Agilent’s TapeStation® 2200.
Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent RNA clean-up in a wide concentration range.
Figure 3. 10 µL of RNA at 50 ng/µL and 5 ng/µL was cleaned up with Omega Bio-tek’s Mag-Bind® TotalPure NGS following manufacturer’s recommended protocols. The RNA was eluted in 20 µL and analyzed on Agilent’s TapeStation® 2200. Recovery rates were 85-92% respectively.
Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-sided size selection on DNA from NGS library prep.
Figure 4. Next-generation sequencing libraries prepared from 350 ng sheared genomic DNA using Kapa Biosystem’s HyperPrep Kits (KK8504) and Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A on the Hamilton Microlab® STAR™. Mag-Bind® TotalPure NGS was used for 2 clean up step (0.8x and 1.0x) following Kapa Biosystems’ recommended protocol for clean up. DNA was analyzed on Agilent’s TapeStation® 2200 following library construction.
Citations
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