我的题目是鸡Mx基因的克隆，我的策略是先根据该基因家族(dynaminfamily)的氨基酸保守区设计简并引物克隆出该基因CDNA的部分片段，然后根据该片段序列设计基因特异性引物，利用RACE技术得到cDNA的3‘和5’末端，然后根据末端序列设计引物扩增出该基因全长cDNA.

我用简并引物从鸡cDNA中扩增出一个片段，约250bp,测序后拿来BLAST时却发现，与之同源性最高的并不是其它的Mx基因，而是人的某一个基因，比对的结果中与该序列同源性较高的有人及鼠Mx基因，但是同源性并不是很高。该基因在同一种生物的不同品种中具有多态性，已经有其它的鸡的Mx基因被克隆，按道理如果该片段是Mx基因的一部分它应该与其它的鸡的Mx基因有很高的同源性才对呀，所以，我怀疑简并引物PCR扩增出来的该片段不是Mx基因，因为RACE试剂盒很贵，现在也不敢继续作下去，怕作下去得不到什么结果。现在贴上我BLAST结果中score排在最前面的部分，还请有经验的兄弟帮我分析分析。我不胜感激。

Sequencesproducingsignificantalignments:(bits)Value

gi|19807872|gb|AC087694.3|Homosapienschromosome8,clone...460.033
gi|19073805|gb|AC090733.7|Homosapienschromosome8,clone...460.033
gi|38081550|ref|XM_358891.1|MusmusculussimilartoMyxovi...440.13
gi|47026513|gb|AC024957.9|Musmusculuschromosome16,clon...440.13
gi|13435314|gb|AC090999.1|CaenorhaBDitiseleganscosmidY8...440.13
gi|21955645|emb|AL732599.5|MouseDNAsequencefromcloneR...440.13
gi|199921|gb|J03368.1|MUSMX2Mouseinterferon-inducedMx2m...440.13
gi|31442540|gb|AC138314.4|MusmusculusBACcloneRP23-218B...420.52
gi|21591808|gb|AC092491.6|Homosapiens12BACRP11-125G9(...420.52
gi|40949625|gb|AC127597.5|MusmusculusBACcloneRP23-71A1...420.52
gi|6996929|ref|NM_010846.1|Musmusculusmyxovirus(influen...402.1
gi|15029783|gb|BC011113.1|Musmusculusmyxovirus(influenz...402.1
gi|13938021|gb|BC007127.1|Musmusculusmyxovirus(influenz...402.1
gi|19705454|ref|NM_134350.1|Rattusnorvegicusmyxovirus(i...402.1
gi|16041423|gb|AC091589.8|Homosapienschromosome18,clon...402.1
gi|47104230|gb|BT012815.1|Lycopersiconesculentumclone11...402.1
gi|20270172|gb|AC114485.2|Homosapienschromosome1clone...402.1
gi|28372591|gb|AC008939.8|Homosapienschromosome5clone...402.1
gi|19310326|gb|AC105250.3|HomosapiensBACcloneRP11-39C1...402.1
gi|37537322|dbj|BS000055.1|Pantroglodyteschromosome22c...402.1
gi|37537321|dbj|BS000054.1|Pantroglodyteschromosome22c...402.1
gi|18464281|gb|AC104690.2|HomosapiensBACcloneRP11-183P...402.1
gi|15321560|gb|AC079232.7|HomosapiensBACcloneRP11-360H...402.1
gi|56724|emb|X52713.1|RNMX3RatmRNAforMx3protein402.1
gi|56722|emb|X52712.1|RNMX2RatmRNAforMx2protein402.1
gi|56720|emb|X52711.1|RNMX1RatmRNAforMx1protein402.1