![Iwai-Chem/Anti-Human CD3 Monoclonal Antibody [OKT3]/Azide Free & Low Endotoxin/E-AB-F1001-Azide Free & Low Endotoxin](images/Iwai-Chem/E-AB-F1001H-FC01__28918.1589411232.jpg)
Product Description
Anti-Human CD3 Monoclonal Antibody
Product Details
Alternate Names | T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E |
Clone No. | OKT3 |
Reactivity | Human |
Host | Mouse |
Uniprot ID | P07766 |
Background | CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. |
Datasheet & Specification (Click link)
Catalog No. | Product Name | Sizes |
E-AB-F10010 | AF/LE Purified Anti-Human CD3 Antibody[OKT3] | 50 μg,500 μg,1 mg |
E-AB-F1001A | Purified Anti-Human CD3 Antibody[OKT3] | 25 μg,100 μg |
E-AB-F1001B | Biotin Anti-Human CD3 Antibody[OKT3] | 25 μg,100 μg |
E-AB-F1001C | FITC Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001D | PE Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001E | APC Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001F | PerCP Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001G | PE/Cyanine5 Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001H | PE/Cyanine7 Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001I | PE/Cyanine5.5 Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001J | PerCP/Cyanine5.5 Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001L | AF488 Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
E-AB-F1001M | AF647 Anti-Human CD3 Antibody[OKT3] | 20 Tests,50 Tests,100 Tests,200 Tests |
References
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by: Elabscience
Questions about this product? Ready to place an order? Email us at orders@iwai-chem.comor give us a call: (650) 486-1541 |
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昨天看了一篇文章,用antisense阻断BMP2(具有抑制凋亡的作用)的表达,不能筛选出稳定转然的克隆,因为发生了细胞凋亡。所以现在很迷茫,请各位老师帮忙!
谢谢!
我想用一个链霉素抗性的载体pCDFDuet™和一个卡纳pet28a或者氨卞pet32a的载体共转化入表达菌株rosetta。有什么可能的问题吗?
目基表达基工程叙述~
2逆转录使用合oligo
3p内参
4使用根据已知序列设计游引物提供游引物做PCR注意设计游引物退火温度与游引物相差
5产物切胶收TA克隆测序
oligo游引物我根据TakaraBD公司manual自设计经我使用效具体操作参照takara3-racemanual先做3-race解熟悉功再做5-race5-race要买试剂盒做较复杂
设计引物候引物度设置25-27左右

