
Product Description
CD4 Monoclonal Antibody
Overview
Synonyms | CD 4,CD4 (L3T4),CD4,CD4 antigen (p55),CD4 antigen,CD4 molecule,CD4 receptor,CD4+ Lymphocyte deficiency,included,CD4,CD4mut,L3T4,Leu3,Ly-4,Lymphocyte antigen CD4,MGC165891,OTTHUMP00000238897,p55,T cell antigen T4,T cell antigen T4/LEU3,T cell differentiation antigen L3T4,T cell OKT4 deficiency,included,T cell surface antigen T4/Leu 3,T cell surface antigen T4/Leu3,T cell surface glycoprotein CD4,T-cell surface antigen T4/Leu-3,T-cell surface glycoprotein CD4,W3/25,W3/25 antigen |
Swissprot | P01730 |
Source | Mouse |
Reactivity | Human,Mouse,Rat |
Immunogen | Synthetic Peptide |
Application | IHC-p(Detection kit: E-IR-R217),IF |
Recommended dilution | IHC 1:100-1:300, IF 1:50-200 |
Concentration | 1 mg/mL |
Clonality | Monoclonal |
Properties
Cellular localization | Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. |
Tissue specificity | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
Clone No. | Clone:9G1 |
Isotype | IgG |
Purification | Protein A purification |
Conjugation | Unconjugated |
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
Storage buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Background | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
Spec Sheet E-AB-22098 (PDF File)
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by: Elabscience
Questions about this product? Ready to place an order? Email us at orders@iwai-chem.comor give us a call: (650) 486-1541 |
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
昨天看了一篇文章,用antisense阻断BMP2(具有抑制凋亡的作用)的表达,不能筛选出稳定转然的克隆,因为发生了细胞凋亡。所以现在很迷茫,请各位老师帮忙!
谢谢!
我想用一个链霉素抗性的载体pCDFDuet™和一个卡纳pet28a或者氨卞pet32a的载体共转化入表达菌株rosetta。有什么可能的问题吗?
目基表达基工程叙述~
2逆转录使用合oligo
3p内参
4使用根据已知序列设计游引物提供游引物做PCR注意设计游引物退火温度与游引物相差
5产物切胶收TA克隆测序
oligo游引物我根据TakaraBD公司manual自设计经我使用效具体操作参照takara3-racemanual先做3-race解熟悉功再做5-race5-race要买试剂盒做较复杂
设计引物候引物度设置25-27左右

