Product Description
| Product Name | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| ACTC1-specific Monoclonal Antibody | E-AB-27018 | 206 | IgG1 | WB,IHC,IF,ELISA* | Human,Mouse | 60μL, 120μL, 200μL |
Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin,the predominant actin isoform in adult heart,which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.
Spec Sheet E-AB-27018 (PDF File)
| Synonyms | ACTC,ACTC1,ACTC1 cardiac muscle 1,ACTC1 specific,Actin cardiac muscle specific,Actin,alpha cardiac muscle 1,actin,alpha,cardiac muscle 1,Alpha cardiac actin,CMD1R,CMH11 |
| Swiss-Prot | P68032 |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Immunogen | Synthetic Peptide |
| Purification Method | Protein G purification |
| Formulation | PBS with 0.02% sodium azide, 50% glycerol, PH7.3 |
| Storage | Store at -20?. Avoid freeze / thaw cycles. |
| Dilution | WB 1:2000-1:20000, IHC 1:100-1:400, IF 1:50-1:500 |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by:Elabscience
ebiomall.com
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昨天看了一篇文章，用antisense阻断BMP2（具有抑制凋亡的作用）的表达，不能筛选出稳定转然的克隆，因为发生了细胞凋亡。所以现在很迷茫，请各位老师帮忙！
谢谢！
我想用一个链霉素抗性的载体pCDFDuet™和一个卡纳pet28a或者氨卞pet32a的载体共转化入表达菌株rosetta。有什么可能的问题吗？
目基表达基工程叙述~
2逆转录使用合oligo
3p内参
4使用根据已知序列设计游引物提供游引物做PCR注意设计游引物退火温度与游引物相差
5产物切胶收TA克隆测序
oligo游引物我根据TakaraBD公司manual自设计经我使用效具体操作参照takara3－racemanual先做3－race解熟悉功再做5－race5－race要买试剂盒做较复杂
设计引物候引物度设置25-27左右
