SMOBIO/[QP2120] Q-PAGE™ Bis-Tris Precast Gel (Mini, 15 wells, 8%), 10 gels/Mini, 15 wells, 8%), 10 gels</span> </li> </ol> </div> <div class=col-sm-3 mb8> <form method=get action=/sho

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SMOBIO/[QP2120] Q-PAGE™ Bis-Tris Precast Gel (Mini, 15 wells, 8%), 10 gels/Mini, 15 wells, 8%), 10 gels</span>
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SMOBIO/[QP2120] Q-PAGE™ Bis-Tris Precast Gel (Mini, 15 wells, 8%), 10 gels/Mini, 15 wells, 8%), 10 gels

品牌 /

SMOBIO

货号 /

QP2120

官网地址

美元价：

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数量：

免费咨询热线

4000-520-616

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Description

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Tris-Glycine gels.

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Key Features

User-friendly gel cassette:
- Numbered and framed wells for sample loading
- Labeled warning sign and green tape as reminder
Enhanced gel performance:
- Enhanced band sharpness
- Better resolution of small proteins
- Stable for shipping at ambient temperature
Easy compatibility:
- Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
- Compatible with most popular protein electrophoresis systems

Storage and stability

Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.

Technical

Clear and sharp bands, high resolution

Q-PAGE™ Bis-Tris Precast Gel shows high resolution of protein separation.

QP2120 Specifications

Gel

Bis-Tris

Buffer systems

MOPS and MES

Features

Clear and sharp bands,

high resolution

Cassette size

Mini Gel

(10 X 8.3 cm)

Gel dimensions

8.1 x 7.4 x 0.1 cm

(W x L x thickness) cm

Electrophoresis system

Bio-Rad systems

Well format &

Capacity

15 wells,

22 μl/well

Gel percentage

8 %

Accessory tray

Production description

Tip card

Gel remover

Cassette opener

Manual

Manual_Q-PAGE™ Bis-Tris Precast Gel, Mini

SDS

SDS_Q-PAGE™ Precast Gel

Migration pattern

Setting Up and Running Q-PAGE™ Mini Precast Gel

Removing Q-PAGE Mini Gel from cassette

Setting up gel/membrane sandwich for Western transfer

Recommendations/Tips for Gel Running

1. Remove comb and tape before adaption. 2. Use fresh 1X running buffer for the inner cathode chamber. 3. Do not use Tris-Glycine running buffer for Q-PAGE™ Bis-Tris Precast Gels. 4. Rinse the wells before sample loading.

Sample Preparation for SDS-PAGE

1. Mix protein sample with 2X sample buffer.  

2. Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.

3. Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)

Prepare Q-PAGE™ for Sample Loading

1.Open the blister tray of Q-PAGE™ Precast Gel.

2.Briefly rinse the gel cassette with ddH2O.

3.Remove tape and comb; avoid squeezing the gel.

4.Adapt Q-PAGE™ to electrophoresis system; instruction are provided below. (BioRad Mini-PROTEAN® Core Electrophoresis System is recommended.)

5.Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.

6.Fill the wells with running buffer prior to sample loading.

7.Load samples and pre-stained protein marker into numbered wells.

8.Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.

Power Setting for Running Q-PAGE™

Optimize the voltage and running time if needed.

130 V
180 V
230 V*2
Running Time*1
45-60 mins
25-40 mins
15-30 mins
Expected Current
Initial (per gel)
Final (per gel)

60-70 mA
20-25 mA

100-110 mA
40-50 mA

130-140 mA
60-70 mA
Expected temperature
25-30°C
25-35 °C
35-45°C

*1 Set voltage higher than 100 V is recommended.

*2 For higher voltage conditions, please use fresh running buffer for inner and outer chambers.

*3 Running time varies depending on gel percentage, running buffer, temperature, and power supply

Remove Q-PAGE™ Gel from Cassette

Open cassette immediately after electrophoresis. Avoid gel drying.

1.Insert the cassette opener into corners of cassette.

2.Sequentially pry the opener to separate the two plates.

3.Gently pull two plates apart from the top of cassette.

4.Carefully detach the gel either from the bottom of gel or the top side of the cassette.

-Avoid diagonally peeling the gel from the corner.

-Use water to help gel detachment if it needed

5.Gently remove the gel for further staining or Western blotting.

Gel Staining Proteins separated using Q-PAGE™ Precast Gels can be further stained with most popular staining reagents, such as Coomassie dyes (R-250 or G-250), Silver-stain solution, and FluoroStain™ Protein Fluorescent Staining Dye. (Cat. No. PS1000)

Transferring Protein from Q-PAGE™ to Blotting Membrane 1. After protein separation using Q-PAGE™, gently detach QPAGE™ from cassette and then equilibrate the gel in transfer buffer. 2. Pre-soak blotting membrane and filter papers in transfer buffer. 3. Assemble transfer sandwich by orientating cathode, sponge, filter papers, gel, membrane, filter papers, sponge, and anode. The protein goes to the direction of cathode to anode. 4. Carefully move roller over the gel/membrane to remove air bubbles and excess buffer until complete contact is established. 5. Insert transfer cassette into transfer module. Notice that black side of cassette should be next to black side of module. 6. Fill transfer tank with pre-cooled transfer buffer to the highest water level. 7. Set constant voltage at 100 V. Transfer for 90 minutes at low temperature condition. Pre-stained protein marker should be visible on the membrane after transfer is completed. Transfer of proteins to the membrane can be checked using Ponceau S staining before blocking step.

Supplemental Information for Using Q-PAGE™ Precast Gel

Adapting Q-PAGE™ Mini Precast Gel to BioRad Mini-PROTEAN® Core 1. After removing comb and tape, place the Q-PAGE™ Mini Precast Gel with notched plate facing toward inner chamber. 2. Align the notched plate to ensure the edge sits just below the notch at the top of green gasket. 3. Gently press gel cassette toward green gasket and then lock gel cassette with two green arms. Avoid squeezing the cassette and gel.

4. Fill inner chamber with running buffer to check tightness of seal. If necessary, reassemble and check the seal again. 5. Fill inner chamber with running buffer to ensure gel wells are completely covered. 6. Fill outer chamber with running buffer to the highest level.

Adapting Q-PAGE™ Mini Precast Gels to other electrophoresis system, please follow the manufacturer’s instruction.

Buffer recipes

2X sample buffer with reducing agent 62.5 mM Tris-HCl pH 6.8, 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)

10X MOPS running buffer 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS, 3.0 g EDTA. Bring up the volume to 1 L with ddH2O.

10X MES running buffer 60.6 g Tris base, 97.6 g MES, 10.0 g SDS, 3.0 g EDTA. Bring up the volume to 1 L with ddH2O.

1X running buffer Dilute 100 ml 10X running buffer with 900 ml ddH2O.

10X transfer buffer 30.0 g Tris base, 144.0 g Glycine. Bring up the volume to 1 L with ddH2O.

1X transfer buffer *Cool 1X transfer buffer to 4°C before using. Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O. **Add SDS to 0.1% to promote transfer of high molecular weight proteins.

Troubleshooting Guidelines

Problem

Possible Cause

Suggested Solution

Well deformation

Pull one side of comb out of cassette.

Smoothly pull the comb straight out of the cassette.

Bubbles between gel and cassette

Gel has been frozen or stored at wrong temperature.

Store Q-PAGE Precast Gels at 4°C.

Buffer leaking from the inner chamber

Untight assembly of gels to the electrode modules

Reassemble Q-PAGE gels into the electrodemodules.

Fill outer chamber with 1X running buffer to thehighest level.

Samples do not sink into the wells.

Residual gel storage buffer in the wells

Rinse the gel wells with ddH2O or 1X running bufferbefore loading.

Insufficient sample buffer

Use more sample buffer to prepare samples.

Current is zero and sample do not migrate into gel

Tape at bottom of gel not removed

Remove tape

Gels run faster or more slowly than expected.

Incorrect running buffer

Check buffer composition.

Use fresh 1X running buffer for inner chamber.

Crooked bands at middle or bottom of gel

Gel has been frozen or stored at wrong temperature.

Store Q-PAGE Precast Gels at 4°C.

Incorrect running buffer

Check buffer composition.

Use fresh 1X running buffer for inner chamber.

Band pattern curves toward one or both sides of gel.

Buffer leaking from the inner chamber

Check assembly of gels into the electrode modules.

Excessive heating of gel

Check buffer composition. Or dilute running bufferto 0.5-0.75X.

Do not exceed recommended running conditions.

Insufficient buffer in inner or outer buffer chamber

Fill inner and outer chambers to completely covergel wells.

Poor resolution or fuzzy bands

Excessive heating of gel

Check buffer composition.

Do not exceed recommended running conditions.

Incorrect running buffer

Check buffer composition.

Bands are missing on the membrane after Westerntransferring.

Proteins move in the wrong direction

Check the order of gel/membrane sandwich assembly,the direction of transfer cassette in transfer modules, and the polarity ofconnections to power supply.

Swirls or missing bands; bands trail off in multipledirections on the membrane after Western transferring.

Contact between the membrane and the gel was poor;Air bubbles or excess buffer remains between the blotting membrane andthe gel.

Use thicker/more filter paper in the gel/membranesandwich

Remove air bubbles and excess buffer betweengel and membrane by carefully moving the roller over the membrane.

Apparent molecular sizes of prestained proteinmarkers are different as indicated.

Prestained protein markers used have not beencalibrated for use with Q-PAGE gels. Dyes for staining protein markers affect themigration patterns of prestained proteins in different buffer systems.

Calibrate prestained protein markers againstunstained proteins of known size or use SMOBIO’s ExcelBand™ Protein Markers.

Q-PAGE™ Precast Gel

Gel Type

Bis-Tris

TGN (Tris-Glycine-Novel)

Buffer systems

MOPS and MES

Tris-Glycine (Laemmli)

Features

Clear and sharp bands, high resolution

Quick running, clear bands

Cassette size

Mini Gel(10 x 8.3 cm)

Midi Gel(10 X 10 cm)

Mini Gel(10 x 8.3 cm)

Midi Gel(10 X 10 cm)

Electrophoresis system

Bio-Rad systems

Mini Gel Tank

Xcell SureLock,

Hoefer SE260

Bio-Rad systems

Mini Gel Tank

Xcell SureLock,

Hoefer SE260

Well format &

Capacity

12 wells, 25 μl/well

15 wells,22 μl/well

12 wells, 40 μl/well

15 wells, 28 μl/well

12 wells, 25 μl/well

15 wells, 22 μl/well

12 wells, 40 μl/well

15 wells, 28 μl/well

Gel percentage/

Cat. No.

10%

QP2110

QP2120

QP3110

QP3120

QP4210

QP4220

QP5210

QP5220

12%

4-15%

QP2310

QP2320

QP3310

QP3320

QP4510

QP4520

QP5510

QP5520

4-12%

QP2510

QP2520

QP3510

QP3520

ExcelBand™ Protein Markers

Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference

YesBlot™ Western Marker I

Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)

FluoroStain™ Protein Fluorescent Staining Dye

Compatible to MASS analysis — compatible to the analysis of mass spectra, such as LC-MS/MS, MALDI-TOF, and etc.
High sensitivity — detection level achieve ~3 ng, similar to silver staining
Substitution of the Coomassie Blue protein staining method

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健那绿——高中唯一一个活体染色剂。染线粒体的，染成蓝绿色

关于核酸染料的若干问题实验方法123

fine082005-07-26

今天看到一个试剂公司的快讯，想换种毒害小些，灵敏度不错的染料！
1、快讯上是Invotrogen公司的SYRBGOLD，不过500ul/1390元，实在有点贵，为了查用量；
2、结果又查出赛百盛的GoldViewTMDNA染料1ml/100元；
3、还有一种在日光下即可看出DNA条带的上海华舜生物工程有限公司的LightBluedye；

为了一个EB，居然不小心查出三种染料，而对这三种染料的评价也褒贬不一，我个人希望找个毒害小些，而且灵敏度还不能低于EB，价格方面也能让老板接受的来。
而对这三个染料，我有三个问题：
1、SYRBGOLD的用量问题，500ul能用到200次吗（我一般一次用30ml的胶，配在胶中）？
2、GoldViewTMDNA染料的成分是否如有些人所说的就是丫啶橙（能举出确切证据吗）？
3、LightBluedye灵敏度如何？能比得过EB吗？

Lumiprobe』动物活体成像用染料|活体成像|性能参数,报价/价格,...123

cnbio62172021-07-22

【求助】酸性染料比色法测定溶出度,随着时间的增加,溶出量减少? ...123

mxyy2021-07-25

一新药，以麻黄总生物碱作溶出方法学研究如下：
取片剂一片，照溶出度测定法（中国药典2000年版二部附录ⅩC第三法），以水250ml为溶剂，转速为每分钟50转，依法操作，分别经15、30、45、60、75、210分钟取溶液滤过，精密量取续滤液5ml于分液漏斗中，加入pH7.4磷酸盐缓冲液5ml，5ml0.3%溴麝香草酚蓝溶液，用15ml氯仿分两次萃取，合并萃取液，加入0.4g无水硫酸钠，照分光光度法（中国药典2000年版二部附录ⅥA），在410nm波长处测得溶出A值。现15、30、45、60、75、210分钟溶出A值分别为0.0413、0.0544、0.0437、0.0479、0.0394、0.0302。（测定吸收度偏小是否不准，有影响。）

请各位站友指教。

DNA重组与转化_实验方法123

丿宏旭丶MaGe灬2018-02-24

高中学的只有健那绿染线粒体的

【求助】酸性染料比色法测定总生物碱含量中遇到的问题123

防风半夏2007-03-31

我在做中药提取物中总生物碱含量测定,用的是酸性染料比色法,两次试验在处理样品时,同样的重量,同样的溶剂量,只是最后在调节PH值时一次用的是氨水调到9-10,一次用的氢氧化钠溶液调到9-10,但是发现用氨水调的溶液测定值明显比氢氧化钠调的要高得多,这是怎么回事呢?有同学说氨水可能有部分也与酸性染料形成络合物了,但是我的供试液是经三氯甲烷萃取,挥掉三氯甲烷,再用无水乙醇定容的,应该不会存在这种可能的.请教各位大侠,这是什么原因呢?

阅读下面语段.完成下题. 金龟子科中有一些贪食树根或农作物根的...123

星月青城2018-01-26

运用了哪些说明方法，有什么作用？新兴的生物循环再生技术将染料及其他材料完全去除，无限循环再生。这和在一定条件下从石油中制造出聚醋原料再焚烧相比，能量消耗量及二氧化碳排出量均可削减80%。而回收的服装可以返回工厂，重新再生为长纤维。这种方法为延...

【分享】酸性染料比色法测定生物碱含量时为什么硫酸钠会变为蓝色？...123

小帅帅5162021-07-21

我在用酸性染料比色法测定生物碱含量时发现加入的无水硫酸钠变为蓝色了,这是为什么啊
而且样品中的无水硫酸钠未变色,而做标准曲线的五个和空白对照的变为蓝色了,请高手指教,多谢!
还有,是否变蓝对测定结果有影响吗?
谢了哈

欢迎你！请下次规范发贴:)

荧光染料标准品_标准品_生化试剂_知道_生物信息网123

matsukoko2021-07-23

求助各位同僚：
体内诊断用的荧光染料类药物，注册报批该走药品注册流程，还是诊断试剂（医疗器械）流程？
咨询了一些专业人士，有说按照药品的，也有说按照生物制剂的（但本身只属于小分子荧光染料，有点不沾边啊）。查询了国家局的文件，大多都是关于体外诊断试剂的，体内的相当少。
在此请教各位，能否给一些意见。最好能附上国家局相应文件。
感激！

生物质燃料的类型有哪些123

小海8792018-03-01

一般分固体成型燃料、气体燃料和液体燃料

【资源】蛋白质、多肽、核酸等生物分子标记染料_问答123

cnbio62172014-05-23

lumiprobeCorporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(ReactiveDye)和SYBRGreenI染料，用于寡核苷酸合成的亚磷酰胺，点击化学和其它试剂。
产品主要应用：点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
N-羟基琥珀酰亚胺酯类活性染料罗丹明类化合物是以氧杂蕙为母体的碱性咕吨染料，由于特殊的结构及相应的荧光特性，使罗丹明类荧光染料成为化学和生物分析领域中研究较为广泛的课题。与其他常用的荧光染料相比，罗丹明类荧光染料具有光稳定性好、对PH不敏感、较宽的波长范围和较高的荧光量子产率等优点，因此被广泛应用在医学、生物学、环境化学等方面，是分析化学和生物技术领域中最常用的荧光染料。
菁染料是性能优良的荧光标记染料，摩尔吸光系数在荧光染料中是最高的。N-羟基琥珀酰亚胺酯是最常用的脂肪氨基标记试剂，广泛用于蛋白质、氨基酸多肽、抗体、核酸及其他生物分子的标记和检测。通过改变次甲基链的长度,可改变其荧光发射波长，每增加一个双键，按照Huoffman规则正好红移约100nm。
菁染料Cy3和Cy5已成为基因芯片的首选荧光标记物；另外,Cy5,Cy5.5和Cy7，Cy7.5的吸收在近红外区背景非常低，是荧光强度最高、最稳定的长波长染料，特别适合于活体小动物体内成像。但由于菁染料,尤其是不对称菁染料的合成副反应多,副产物极性相近,产物的分离提纯相当困难。菁染料特别是水溶性菁染料分子极性大,分离提纯越加困难。Lumiprobe供应脂溶性和水溶性菁染料。
相关产品：
产品分子结构可替代染料编号：AGF1371A
6-ROX-N-羟基琥珀酰亚胺酯
ROXNHSester,pure6-isomer[img]/KindEditor_4.0.1/attached/image/20130704/20130704102819_7906.jpg[/img]AlexaFluor568编号：AGF1326A
Cy3-N-羟基琥珀酰亚胺酯
Cy3NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103024_8531.jpg[/img]AlexaFluor546NHSester
DyLight549NHSester
编号：AGF1330A
Cy3.5-N-羟基琥珀酰亚胺酯
Cy3.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103242_2437.jpg[/img]AlexaFluor594NHSester
DyLight594NHSester
编号：AGF1338A
Cy5-N-羟基琥珀酰亚胺酯
Cy5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103422_4468.jpg[/img]AlexaFluor647NHSester
DyLight649NHSester
编号：AGF1345A
Cy5.5-N-羟基琥珀酰亚胺酯
Cy5.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103557_5875.jpg[/img]AlexaFluor680NHSester
DyLight680NHSester
编号：AGF1349A
Cy7-N-羟基琥珀酰亚胺酯
Cy7NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103713_8843.jpg[/img]编号：AGF1356A
Cy7.5-N-羟基琥珀酰亚胺酯
Cy7.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103756_5406.jpg[/img]编号：AGF1374A
磺酸基-Cy3-N-羟基琥珀酰亚胺酯
Sulfo-Cy3NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103830_1656.jpg[/img]AlexaFluor546
DyLight549
编号：AGF1377A
磺酸基-Cy5-N-羟基琥珀酰亚胺酯
Sulfo-Cy5NHSester[img]/KindEditor_4.0.1/attached/image/20130705/20130705095752_6656.jpg[/img]AlexaFluor647
DyLight649
编号：AGF1379A
磺酸基-Cy7-N-羟基琥珀酰亚胺酯
Sulfo-Cy7NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704104047_6968.jpg[/img]相似系列产品：
羰基活性荧光染料
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羧酸类染料

高中生物实验所有染色剂及其性质详解(完整版)123

枫岛LO03392018-02-18

1、菲林试剂还原糖砖红色沉淀（需水域）
2、苏丹三脂肪橙红
3、苏丹四脂肪红
4、双缩脲蛋白质紫
5、龙胆紫染色质紫
6、碘淀粉蓝
7、健那绿线粒体绿
8、甲基绿 DNA 绿
9、吡罗红 RNA 红
10、溴麝香草酚蓝 CO2 由蓝变绿再变黄
11、重铬酸钾酒精酸性条件下由橙色变成灰绿
12、醋酸洋红（龙胆紫、改良苯酚品红）染色质红
13、台盼蓝检验活死细胞死细胞会被染成蓝色（不常用）