![SMOBIO/[QP2120] Q-PAGE™ Bis-Tris Precast Gel (Mini, 15 wells, 8%), 10 gels/Mini, 15 wells, 8%), 10 gels</span>
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Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Tris-Glycine gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced band sharpness
Better resolution of small proteins
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Clear and sharp bands, high resolution
Q-PAGE™ Bis-Tris Precast Gel shows high resolution of protein separation.
QP2120 Specifications
Gel | Bis-Tris | |
Buffer systems | MOPS and MES | |
Features | Clear and sharp bands, high resolution | |
Cassette size | Mini Gel (10 X 8.3 cm) | |
Gel dimensions
| 8.1 x 7.4 x 0.1 cm (W x L x thickness) cm | |
Electrophoresis system | Bio-Rad systems | |
Well format & Capacity | 15 wells, 22 μl/well | |
Gel percentage | 8 % | |
Accessory tray | Production description Tip card Gel remover Cassette opener | |
Manual
Manual_Q-PAGE™ Bis-Tris Precast Gel, Mini
SDS
SDS_Q-PAGE™ Precast Gel
Migration pattern
Setting Up and Running Q-PAGE™ Mini Precast Gel
Removing Q-PAGE Mini Gel from cassette
Setting up gel/membrane sandwich for Western transfer
Recommendations/Tips for Gel Running
1. Remove comb and tape before adaption. 2. Use fresh 1X running buffer for the inner cathode chamber. 3. Do not use Tris-Glycine running buffer for Q-PAGE™ Bis-Tris Precast Gels. 4. Rinse the wells before sample loading.
Sample Preparation for SDS-PAGE
1. Mix protein sample with 2X sample buffer.
2. Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.
3. Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)
Prepare Q-PAGE™ for Sample Loading
1.Open the blister tray of Q-PAGE™ Precast Gel.
2.Briefly rinse the gel cassette with ddH2O.
3.Remove tape and comb; avoid squeezing the gel.
4.Adapt Q-PAGE™ to electrophoresis system; instruction are provided below. (BioRad Mini-PROTEAN® Core Electrophoresis System is recommended.)
5.Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.
6.Fill the wells with running buffer prior to sample loading.
7.Load samples and pre-stained protein marker into numbered wells.
8.Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.
Power Setting for Running Q-PAGE™
Optimize the voltage and running time if needed.
| 130 V | 180 V | 230 V*2 |
Running Time*1 | 45-60 mins | 25-40 mins | 15-30 mins |
Expected Current Initial (per gel) Final (per gel) |
60-70 mA 20-25 mA |
100-110 mA 40-50 mA |
130-140 mA 60-70 mA |
Expected temperature | 25-30°C | 25-35 °C | 35-45°C |
*1 Set voltage higher than 100 V is recommended.
*2 For higher voltage conditions, please use fresh running buffer for inner and outer chambers.
*3 Running time varies depending on gel percentage, running buffer, temperature, and power supply
Remove Q-PAGE™ Gel from Cassette
Open cassette immediately after electrophoresis. Avoid gel drying.
1.Insert the cassette opener into corners of cassette.
2.Sequentially pry the opener to separate the two plates.
3.Gently pull two plates apart from the top of cassette.
4.Carefully detach the gel either from the bottom of gel or the top side of the cassette.
-Avoid diagonally peeling the gel from the corner.
-Use water to help gel detachment if it needed
5.Gently remove the gel for further staining or Western blotting.
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Q-PAGE™ Precast Gel
Gel Type | Bis-Tris | TGN (Tris-Glycine-Novel) | ||||||
Buffer systems | MOPS and MES | Tris-Glycine (Laemmli) | ||||||
Features | Clear and sharp bands, high resolution | Quick running, clear bands | ||||||
Cassette size | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | ||||
Electrophoresis system | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | ||||
Well format & Capacity | 12 wells, 25 μl/well | 15 wells,22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well |
Gel percentage/ Cat. No. | 8% | 8% | 8% | 8% | 10% | 10% | 10% | 10% |
QP2110 | QP2120 | QP3110 | QP3120 | QP4210 | QP4220 | QP5210 | QP5220 | |
12% | 12% | 12% | 12% | 4-15% | 4-15% | 4-15% | 4-15% | |
QP2310 | QP2320 | QP3310 | QP3320 | QP4510 | QP4520 | QP5510 | QP5520 | |
4-12% | 4-12% | 4-12% | 4-12% |
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QP2510 | QP2520 | QP3510 | QP3520 |
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ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
YesBlot™ Western Marker I
Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)
FluoroStain™ Protein Fluorescent Staining Dye
Compatible to MASS analysis — compatible to the analysis of mass spectra, such as LC-MS/MS, MALDI-TOF, and etc.
High sensitivity — detection level achieve ~3 ng, similar to silver staining
Substitution of the Coomassie Blue protein staining method
ebiomall.com
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健那绿——高中唯一一个活体染色剂。染线粒体的，染成蓝绿色
产品主要应用：点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。
相关产品如下：
中文名英文名产品编号分子结构Cy7.5胺Cy7.5amineAGF1350A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110804_5250.jpg[/img]Cy5胺Cy5amineAGF1332A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110715_7750.jpg[/img]相似系列产品：
抗体、核酸、蛋白质等生物分子标记染料
羰基活性荧光染料
巯基反应性染料
羧酸类染料
二苯胺
甲基绿派洛宁
还有一种忘了。
假期没法联系老师呵呵。
这三种的作用都是什么。
分子生物学实验的染料主要涉及到核酸染料和蛋白质染料.核酸染料主要有EB（溴化乙锭,高致癌性）,goldview,sybr green（实时定量PCR时常用染料）.这些染料可以和核酸双链分子特异性结合发出强荧光而被检测到.蛋白质染料最常用的是考马斯亮蓝 R-250,硝酸银.其中硝酸银有时也用于核酸染色.
染料分为天然染料和人工染料两种。天然染料有胭脂虫红、地衣素、石蕊和苏木素等，它们多从植物体中提取得到，其成分复杂，有些至今还未搞清楚。目前主要采用人工染料，也称煤焦油染料，多从煤焦油中提取获得，是苯的衍生物。多数染料为带色的有机酸或碱类，难溶于水，而易溶于有机溶剂中。为使它们易溶于水，通常制成盐类。
染料可按其电离后染料离子所带电荷的性质，分为酸性染料、碱性染料、中性（复合）染料和单纯染料四大类。 标本干燥后即进行固定，固定的目的有三个：
1）杀死微生物，固定细胞结构。
2）保证菌体能更牢的粘附在载玻片上，防止标本被水冲洗掉。
3）改变染料对细胞的通透性，因为死的原生质比活的原生质易于染色。
产品主要应用：点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
菁染料是性能优良的荧光标记染料，摩尔吸光系数在荧光染料中是最高的。N-羟基琥珀酰亚胺酯是最常用的脂肪氨基标记试剂，广泛用于蛋白质、氨基酸多肽、抗体、核酸及其他生物分子的标记和检测。通过改变次甲基链的长度,可改变其荧光发射波长，每增加一个双键，按照Huoffman规则正好红移约100nm。
菁染料Cy3和Cy5已成为基因芯片的首选荧光标记物；另外,Cy5,Cy5.5和Cy7，Cy7.5的吸收在近红外区背景非常低，是荧光强度最高、最稳定的长波长染料，特别适合于活体小动物体内成像。但由于菁染料,尤其是不对称菁染料的合成副反应多,副产物极性相近,产物的分离提纯相当困难。菁染料特别是水溶性菁染料分子极性大,分离提纯越加困难。Lumiprobe供应脂溶性和水溶性菁染料。
相关产品：
产品分子结构可替代染料编号：AGF1371A
6-ROX-N-羟基琥珀酰亚胺酯
ROXNHSester,pure6-isomer[img]/KindEditor_4.0.1/attached/image/20130704/20130704102819_7906.jpg[/img]AlexaFluor568编号：AGF1326A
Cy3-N-羟基琥珀酰亚胺酯
Cy3NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103024_8531.jpg[/img]AlexaFluor546NHSester
DyLight549NHSester
编号：AGF1330A
Cy3.5-N-羟基琥珀酰亚胺酯
Cy3.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103242_2437.jpg[/img]AlexaFluor594NHSester
DyLight594NHSester
编号：AGF1338A
Cy5-N-羟基琥珀酰亚胺酯
Cy5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103422_4468.jpg[/img]AlexaFluor647NHSester
DyLight649NHSester
编号：AGF1345A
Cy5.5-N-羟基琥珀酰亚胺酯
Cy5.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103557_5875.jpg[/img]AlexaFluor680NHSester
DyLight680NHSester
编号：AGF1349A
Cy7-N-羟基琥珀酰亚胺酯
Cy7NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103713_8843.jpg[/img]编号：AGF1356A
Cy7.5-N-羟基琥珀酰亚胺酯
Cy7.5NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103756_5406.jpg[/img]编号：AGF1374A
磺酸基-Cy3-N-羟基琥珀酰亚胺酯
Sulfo-Cy3NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704103830_1656.jpg[/img]AlexaFluor546
DyLight549
编号：AGF1377A
磺酸基-Cy5-N-羟基琥珀酰亚胺酯
Sulfo-Cy5NHSester[img]/KindEditor_4.0.1/attached/image/20130705/20130705095752_6656.jpg[/img]AlexaFluor647
DyLight649
编号：AGF1379A
磺酸基-Cy7-N-羟基琥珀酰亚胺酯
Sulfo-Cy7NHSester[img]/KindEditor_4.0.1/attached/image/20130704/20130704104047_6968.jpg[/img]相似系列产品：
羰基活性荧光染料
氨基类染料
巯基反应性染料
羧酸类染料
前一阵子实验室开始使用一种新的核酸染料-goldview,取代原先用的溴化乙锭-EB.goldview现在是由赛百盛出售.以下摘自官方网站:
GoldViewTM核酸染料——使用说明
概述
GoldViewTM是一种可代替溴化乙锭（EB）的新型核酸染料，采用琼脂糖电泳检测DNA时，GoldViewTM与核酸结合后能产生很强的荧光信号，其灵敏度与EB相当，使用方法与之完全相同。在紫外透射光下双链DNA呈现绿色荧光，而单链DNA呈红色荧光。GoldView不仅能染DNA，也可用于染RNA。
通过Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠睾丸精母细胞染色体畸变试验，致突变性结果均为阴性；而溴化乙锭（EB）是一种强致癌剂。因此用GoldviewTM代替EB不失为一种明智的选择。
使用方法
1.将100ml琼脂糖凝胶溶液（浓度一般为0.8%~2%）在微波炉中融化。
2.加入5µlGoldView，轻轻摇匀，避免产生气泡。
3.冷却至不烫手时倒胶，待琼脂糖凝胶完全凝固后上样电泳。
4.电泳完毕在紫外灯下观察。若使用数码相机照像记录，则关闭相机的闪光灯，放在自动档即可；若使用凝胶成像系统照相，通过调节光圈、曝光时间，选择合适的滤光片，可得到成像清晰、背景较低的照片。
注意事项
1.胶厚度不宜超过0.5cm，胶太厚会影响检测的灵敏度。
2.加入GoldView的琼脂糖凝胶反复融化可能会对核酸检测的灵敏度产生一定影响，但不明显。
3.通过凝胶电泳回收DNA片段时，建议使用GoldView染色，在自然光下切割DNA条带，避免紫外线与EB对目的DNA产生的损伤，可明显提高克隆、转化、转录等分子生物学下游操作的效率。
4.虽然未发现GoldView有致癌作用，但对皮肤、眼睛会有一定的刺激，操作时应戴上手套。
电泳结果显示GV灵敏度与EB相当
问题1.Goldview到底是不是丫啶橙？
2.赛百盛不公布其成分的原因是害怕其为丫啶橙还是出于技术保密？
而且样品中的无水硫酸钠未变色,而做标准曲线的五个和空白对照的变为蓝色了,请高手指教,多谢!
还有,是否变蓝对测定结果有影响吗?
谢了哈
欢迎你！请下次规范发贴:)
取片剂一片，照溶出度测定法（中国药典2000年版二部附录ⅩC第三法），以水250ml为溶剂，转速为每分钟50转，依法操作，分别经15、30、45、60、75、210分钟取溶液滤过，精密量取续滤液5ml于分液漏斗中，加入pH7.4磷酸盐缓冲液5ml，5ml0.3%溴麝香草酚蓝溶液，用15ml氯仿分两次萃取，合并萃取液，加入0.4g无水硫酸钠，照分光光度法（中国药典2000年版二部附录ⅥA），在410nm波长处测得溶出A值。现15、30、45、60、75、210分钟溶出A值分别为0.0413、0.0544、0.0437、0.0479、0.0394、0.0302。（测定吸收度偏小是否不准，有影响。）
请各位站友指教。
2、苏丹三 脂肪 橙红
3、苏丹四 脂肪 红
4、双缩脲 蛋白质 紫
5、龙胆紫 染色质 紫
6、碘 淀粉 蓝
7、健那绿 线粒体 绿
8、甲基绿 DNA 绿
9、吡罗红 RNA 红
10、溴麝香草酚蓝 CO2 由蓝变绿再变黄
11、重铬酸钾 酒精 酸性条件下由橙色变成灰绿
12、醋酸洋红（龙胆紫、改良苯酚品红） 染色质 红
13、台盼蓝 检验活死细胞 死细胞会被染成蓝色（不常用）
1、快讯上是Invotrogen公司的SYRBGOLD，不过500ul/1390元，实在有点贵，为了查用量；
2、结果又查出赛百盛的GoldViewTMDNA染料1ml/100元；
3、还有一种在日光下即可看出DNA条带的上海华舜生物工程有限公司的LightBluedye；
为了一个EB，居然不小心查出三种染料，而对这三种染料的评价也褒贬不一，我个人希望找个毒害小些，而且灵敏度还不能低于EB，价格方面也能让老板接受的来。
而对这三个染料，我有三个问题：
1、SYRBGOLD的用量问题，500ul能用到200次吗（我一般一次用30ml的胶，配在胶中）？
2、GoldViewTMDNA染料的成分是否如有些人所说的就是丫啶橙（能举出确切证据吗）？
3、LightBluedye灵敏度如何？能比得过EB吗？
