ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta
- Product No.SEA026Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 37pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 454 For more details, please contact local distributors!US$ 648 For more details, please contact local distributors!US$ 2916 For more details, please contact local distributors!US$ 5508 For more details, please contact local distributors!US$ 45360 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1).No significant cross-reactivity or interference between Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and analogues was observed.
Recovery of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
Matrices listed below were spiked with certain level of recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-98 | 94 |
| EDTA plasma(n=5) | 93-102 | 96 |
| heparin plasma(n=5) | 84-102 | 95 |
Precision of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 92-101% | 98-105% | 90-97% |
| EDTA plasma(n=5) | 92-99% | 85-99% | 87-101% | 98-105% |
| heparin plasma(n=5) | 80-104% | 95-102% | 91-103% | 97-104% |
Stability of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA026Mu02 | Recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA026Mu01 | Recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA026Mu02 | Polyclonal Antibody to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | WB; IHC; ICC; IP. |
| PAA026Mu01 | Polyclonal Antibody to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | WB; IHC; ICC; IP. |
| SEA026Mu | ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA026Mu | CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | Chemiluminescent immunoassay for Antigen Detection. |
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大家都用过哪些DNA提取试剂盒，能不能推荐几种
哪个正规生物公司DNA提取试剂好用，纯，效果好
这个与琼脂糖电泳有关。提取的DNA分子量很大，可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道，象那个一万与一万五和条带差别很近。
能有效的处理土壤样品中一些很难处理的组分如： 真细菌孢子（eubacterial spores）、内芽孢（endospores）、格兰仕阳性菌（gram positive bacteria）、酵母（yeast）、线虫（nematodes）、海藻（algea）、真菌（fungi）等。2. 样品处理过程中使用的 MT Buffer和Sodium Phosphate Buffer可在整个提取过程中有效的保护核酸，并最大限度的降低RNA污染。3. 基于硅珠的纯化方法，可有效地去除腐植酸、多元酚等PCR抑制物4. 纯化所得的DNA可直接用于PCR、酶切都后续实验之中。5. 对腐植酸含量特别高的样品，附加额外的处理方法。注：对于去除腐植酸的方法在使用以下任何一种方法之前，请预先准备5.5M的异硫氰酸胍（Guanidine Thiocyanate）溶液方法A：1.当操作到土壤DNA提取试剂盒的第九步时，使用14,000 x g (~约5秒)的短时离心替代硅珠的自然沉降，移除上清。2.用1ml事先准备好的异硫氰酸胍溶液洗涤、重悬硅珠。3.14,000 x g (~约5秒)短时离心该离心管，除去上清。4.重复上述的洗涤步骤，直到硅珠恢复到原先的颜色。5.最后一次洗涤之后，用1ml的异硫氰酸胍溶液重悬硅珠，将600μl重悬液转移到SPIN filter里，14,000 x g离心1分钟。6.移除接液管中的废液，将剩余的重悬液转移至SPIN filter里，14,000 x g离心1分钟，弃去废液。7.从提取试剂盒操作说明的第十二步开始继续操作。方法B：1.将下列成分混合于1.5ml的离心管中，组成腐植酸洗涤液：978 μl Sodium Phosphate Buffer122 μl MT Buffer250 μl PPS2.充分混匀后，全速离心1分钟，将上清转移到一个新的离心管中（容量大于或等于2ml）3.加入等体积的5.5M的异硫氰酸胍溶液、混匀。4.完成了土壤试剂盒操作说明中的第九步后，将500μl的腐植酸洗涤液加入SPIN filter5.14,000 x g离心1分钟，弃去废液。6.重复上述的洗涤步骤，直到硅珠恢复到原先的颜色。7.从提取试剂盒操作说明的第十二步开始继续操作。
