- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Introduction
Adenosine diphosphate (ADP) is an organic molecule which plays a central role in cellular metabolism and energy transfer reactions. ADP is a product of ATP de-phosphorylation and can be rephosphorylated to ATP or further de-phosphorylation to AMP. This cycling of ADP is central to transferring potential (thermodynamic) energy from one source to another. Besides its involvement in glycolysis, citric acid cycle and oxidative phosphorylation, ADP is also stored as dense bodies in blood platelets. Upon platelet activation, ADP is release which leads to further platelet activation through APD receptors (1-8).
Cell Technology’s Fluoro ADP assay provides a reliable, sensitive fluorimetric assay for the quantification of ADP in biological samples.
Key Benefits
- Detection of ADP in cells or tissue extracts.
- Detection of ADP in cell death, energy metabolism, mitochondria function.
- ADP measurement in ATP consuming enzymes such as Kinases and ATPases.
- ADP detection in Bacterial, Fungal and Plant Cells.
- Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm).
Additional information
| Kit Size | 100, 500 |
|---|
The Fluoro ADP detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence ADP and a coupled enzyme reaction to produce its fluorescent analog. There is a linear relationship of ADP concentration to the fluorescent analog concentration. An ADP standard curve is generated to interpolate sample ADP concentrations. The kit can be used in both endpoint and kenitic modes.
Reaction:
1. ADP + Enzyme Coupled Reaction + Non-Fluorescent Detection Reagent → Fluorescent Analog + AMP
Detection: Excitation: 530-570nm and Emission at 590-600nm
2. 50µL of sample or ADP Standard
+
50µL of Enzyme Reaction Cocktail
↓
Incubate 30 – 60 minutes; RT; DARK
Read on Plate Reader: Excitation: 530-570nm
Emission at 590-600nm
Figure 1. ADP vs ATP Standard Curve fitted with linear regression.
ADP mM Spike | % Recovery |
50 | 93.08 |
12.5 | 92.04 |
| 1 | 118.48 |
Table 1. ADP was spiked into Jurkat cell samples. % Recovery was determined via linear regression from ADP standard curve. N=3 per sample.
| Document Title |
| Fluoro ADP Protocol |
| Fluoro ADP Datasheet |
| msds.fluoroADP |
ebiomall.com
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A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
刚入这行，谢谢大家
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
