| BMS 309403FABP4 inhibitor,potent and selective |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
- View current batch:
- Purity = 98.14%
- COA (Certificate Of Analysis)
- HPLC
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Related Biological Data
Related Biological Data
| Cell experiment [1]: | |
Cell lines | αP2+/+ and αP2-/- THP-1 monocytic leukaemia cell line |
Preparation method | The solubility of this compound in DMSO is >18.15mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 1-25 μM |
Applications | Treatment with BMS309403 significantly decreased MCP-1 production from THP-1 macrophages in a dose- and time-dependent manner. |
| Animal experiment [1]: | |
Animal models | Male Apoe-/- mice in the C57BL/6J background |
Dosage form | oral gavage, 15 mg/kg/d, 6 weeks |
Application | BMS-309403 significantly reduced the extent of atherosclerotic lesion area in the proximal aorta in both the early and late intervention studies. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Furuhashi M, Tuncman G, Grgün C Z, et al. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2[J]. Nature, 2007, 447(7147): 959. | |
BMS 309403 Dilution Calculator
calculate
BMS 309403 Molarity Calculator
calculate
| Cas No. | 300657-03-8 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | 2-((2"-(5-ethyl-3,4-diphenyl-1H-pyrazol-1-yl)-[1,1"-biphenyl]-3-yl)oxy)acetic acid | ||
| Canonical SMILES | OC(COC1=CC(C2=CC=CC=C2N3N=C(C4=CC=CC=C4)C(C5=CC=CC=C5)=C3CC)=CC=C1)=O | ||
| Formula | C31H26N2O3 | M.Wt | 474.55 |
| Solubility | ≥18.15mg/mL in DMSO | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
BMS309403 is specifically designed to target FABP4 with a Ki value less than 2 nM. It is a potent and selective FABP4 inhibitor.[1]
Fatty acid binding proteins (FABPs) are small-molecular weight hydrophobic proteins containing a large hydrophobic cavity, into which naturally occurring long-chain fatty acids and synthetic hydrophobic ligands can be accepted. FABPs act as transporters of endogenous fatty acids from the cell surface to various sites of fatty acid storage and metabolism. In addition to the roles of FABP4 in regulating lipid metabolism and insulin sensitivity, recent pharmacological and biological findings have indicated a regulatory function of FABP4 in inflammation. FABP4 is expressed mainly to macrophages and inflammatory response[1,2]
BMS309403 is an aromatic biphenyl azol compound that competes with fatty acids for the binding pocket of A-FABP with high specificity. BMs30940323 has been shown to lower Mcp-1 secretion from thp-1 macrophages.[3,4]
Chronic administration of BMS309403 (15 mg/kg/day; from 12 to 18 weeks of age) in ApoE-/-mice significantly improved therelaxations, maximal relaxation and EC50 to UK14304, acetylcholine and A23187. Mice orally BMS309403 significantly increased glucose uptake in myotubes in a time and dose dependent manner. Administered with BMS309403 are effectively protected against severe atherosclerosis and type 2 diabetes.[3,4]
References:[1]Okada T, Hiromura M, Otsuka M etal. , Synthesis of BMS-309403-related compounds, including [¹⁴C]BMS-309403, a radioligand for adipocyte fatty acid binding protein. Chem Pharm Bull (Tokyo). 2012;60(1):164-8.[2]Suhre K, Römisch-Margl W, de Angelis MH etal. , Identification of a potential biomarker for FABP4 inhibition: the power of lipidomics in preclinical drug testing. J Biomol Screen. 2011 Jun;16(5):467-75[3] Lin W1, Huang X, Zhang Letal. , BMS309403 stimulates glucose uptake in myotubes through activation of AMP-activated protein kinase. PLoS One. 2012;7(8):e44570.[4] Lee MY, Li H, Xiao Y, Zhou Z, Xu A, Vanhoutte PM. Chronic administration of BMS309403 improves endothelial function in apolipoprotein E-deficient mice and in cultured human endothelial cells. Br J Pharmacol. 2011 Apr;162(7):1564-76.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
1、DNA提取问题：现在核算提取试剂盒大部分都是吸附柱法,相信你应该不会操作错误,如果提取之后进行核酸定量结果很低的话,试着再最后一步溶解核算的时候把水稍微加温（37℃就可以了）,溶解时间稍微长一些,然后再离心的时候采用分次离心,比如开始你用200ul溶解的话,你可以分两次用100ul水溶解离心,这样可以提高DNA的产率.
2、电泳问题：如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
buffer P1：除去RNA
buffer P2：裂解细胞
buffer P3：沉淀DNA
buffer WA、buffer WB：都是洗涤液（这两个之间有什么区别我也不清楚）
TE：溶解DNA.
哪个正规生物公司DNA提取试剂好用，纯，效果好
大家都用过哪些DNA提取试剂盒，能不能推荐几种
而且比较容易买到，同时提取的DNA便于纯化
