
Description
Product Highlights
- Fast – simple extraction of high purity total RNA from twelve samples, ideal for use in all applications, in as little as 40 minutes
- High-performance – excellent recovery of high quality RNA from single cells, laser-captured or flow-sorted cells, cryosections and small tissue samples, including biopsies
- Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation
- Concentrated - small elution volumes ensure reaction volumes can be kept low in downstream applications, giving increased reaction efficiency
- Convenient – includes all necessary components, including filters (shredders) and DNase I
Product Description
The ISOLATE II RNA Micro Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol.
By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Micro Kit provides a fast method for the purification of high-quality total RNA from single cells, pellets of cultured cells, cryosections, laser-captured or flow sorted cells and small amounts of tissues, for example biopsy materials.
For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed usinga convenient on-column DNase treatment with the RNase-free DNase I that is supplied with the kit.
The ISOLATE II RNA Micro Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Micro Kit can be used to purify samples prior to PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.
Applications
- RT-qPCR
- End-point RT-PCR
- Northern, dot and slot blotting
- Array analysis
- RNA protection assays
- RNA-Seq

ISOLATE II RNA Micro Kit Customer Review
Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchISOLATE II Selection Chart
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1、DNA提取问题:现在核算提取试剂盒大部分都是吸附柱法,相信你应该不会操作错误,如果提取之后进行核酸定量结果很低的话,试着再最后一步溶解核算的时候把水稍微加温(37℃就可以了),溶解时间稍微长一些,然后再离心的时候采用分次离心,比如开始你用200ul溶解的话,你可以分两次用100ul水溶解离心,这样可以提高DNA的产率.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
大家都用过哪些DNA提取试剂盒,能不能推荐几种
这个与琼脂糖电泳有关。提取的DNA分子量很大,可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道,象那个一万与一万五和条带差别很近。
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.

