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分子实验方法6: 基因组DNA的提取 实验方法
点击量 173 更新于 2021-09-23
第六章 基因组DNA的提取第一节 概 述  基因组DNA的提取通常用于构建基因组文库、Southern杂交(包括RFLP)及PCR分离基因等。利用基因组DNA较长的特性,可以将其与细胞器或质粒等小分子DNA分离。
DNA Extraction from Cheek Cells 实验方法
点击量 352 更新于 2021-09-21
Extraction from Cheek Cells DNA Sample Preparation Cheek cells are obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for
DNA提取沉淀法,DNA extraction precipitation method,音标,读音...
点击量 211 更新于 2021-09-17
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国际化学品安全卡(中文版)
点击量 179 更新于 2021-09-16
Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is
免疫共沉淀_CoIP原理_免疫共沉淀实验优化方案
点击量 179 更新于 2021-09-15
DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl , 10 mM EDTA, 50 mM Tri
PreparationofSonicatedHumanDNA
点击量 138 更新于 2021-09-14
Preparation of Sonicated Salmon Sperm DNA1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps, weigh 0.25 g/50 ml conical and add 50 ml/conical
丙二醛(MDA)的测定方法
点击量 187 更新于 2021-09-13
DNAFromWholeBloodforPCR(fromHiguchi,R.(1989)Amplifications2:1-3)Obtain65-100µlofbloodbyretro-orbitalbleedwithaheparinizedmicrocapillarytube.Expelbloodimmediatelyintoa1.5mlmicrofugetubecontaining20µlof10mMEDTA.Miximmediatelytopreventclotformati
免疫共沉淀(CoIP)_图文
点击量 163 更新于 2021-09-11
Author:Laura-LeeBoodramSource:Laura-LeeBoodram,DepartmentofLifeSciences,TheUniversityoftheWestIndiesAbstract:Theprotocolissimpleandfairlyrapid.Itdoesnotrequiretheuseoforganicsolventsbutratherutilizessaltextractiontoprecipitatecontaminatingproteins.Highqua
【盘点二】2020年度FDA审批通过的抗肿瘤小分子抑制剂
点击量 139 更新于 2021-09-09
ES CELL DNA EXTRACTION: TUBE METHOD Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover m
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